The anti-obesity property of bitter melon powder (BMP) was investigated using a high-fat diet (HFD) obesity model. Obese rats were fed a HFD and treated with BMP for 8 weeks. We observed that BMP could effectively retard the body weight gain and improve the insulin sensitivity compared to the HFD group (p < .05). BMP also showed reduced endotoxin load as lipopolysaccharide-binding protein (LBP) level in the system. 16S rRNA sequencing indicated that BMP dramaticlly increased the relative abundance of Blautia and Allobaculum at the genus level. Metabolomic data revealed that dietary BMP changed metabolic profiles, including the metabolism of organic acids, amino acids and sugars. Additionally, some significant correlations was observed between specific gut microbiota at the genus level and effective biomarkers. These results reveal that the inhibition of insulin resistance and anti-inflammatory effect of BMP in obesity are illustrated with the restoration of gut microbiota and gut-generated metabolites.

使用高脂饮食（HFD）肥胖模型研究了苦瓜粉（BMP）的抗肥胖特性。给肥胖大鼠喂食HFD并用BMP治疗8周。我们观察到，与HFD组相比，BMP可以有效地抑制体重增加并改善胰岛素敏感性（p  <.05）。BMP还表现出减少的内毒素负荷，即系统中的脂多糖结合蛋白（LBP）水平。16S rRNA测序表明BMP显着增加了Blautia和Allobaculum的相对丰度在属水平上。代谢组学数据显示，饮食中的BMP改变了代谢谱，包括有机酸，氨基酸和糖的代谢。另外，在属水平的特定肠道菌群与有效生物标志物之间观察到一些显着的相关性。这些结果表明，通过肠道微生物群和肠道产生的代谢产物的恢复可以说明肥胖中BMP对胰岛素抵抗的抑制作用和抗炎作用。