Butyrate produced by microbial fermentation of dietary fibres beneficially affect the colonic epithelium. Here we enhanced butyrate production through in vitro incubations of different sources of arabinoxylan (AX) with butyrogenic bacteria (Butyrivibrio fibrisolvens, Eubacterium rectale) to explore their potential synbiotic effects on the intestinal epithelial barrier function. B. fibrisolvens incubated with AX produced the highest butyrate levels (15 mM). Sodium-butyrate (1–10 mM) and fermentation products (5% v/v) from B. fibrisolvens increased the barrier function in a human colonic epithelial cell line. This was associated with regulation of different genes involved in epithelial integrity, mucus secretion, apoptosis, oxidative stress, and butyrate transport. In conclusion, butyrate in concentrations that can be achieved by dietary intervention in vivo enhanced the epithelial barrier function in vitro. B. fibrisolvens might be a potential new probiotic for increasing colonic butyrate production in humans, specifically in synbiotic combination with AX, a common DF component of food cereals.

通过膳食纤维的微生物发酵产生的丁酸酯有利地影响结肠上皮。在这里，我们通过将不同来源的阿拉伯木聚糖（AX）与产热细菌（Butyrivibrio fibrisolvens， Eubacterium rectale）进行体外温育来提高丁酸生产，以探索它们对肠道上皮屏障功能的潜在合生作用。与AX一起温育的纤维双歧杆菌产生最高的丁酸水平（15 mM）。纤维溶解双歧杆菌的丁酸钠（1-10 mM）和发酵产物（5％v / v）增加了人类结肠上皮细胞系的屏障功能。这与涉及上皮完整性，粘液分泌，凋亡，氧化应激和丁酸转运的不同基因的调节有关。总之，体内饮食干预可达到的丁酸浓度可增强体外的上皮屏障功能。纤溶双歧杆菌可能是增加人体内结肠丁酸产量的潜在新益生菌，特别是在与食品谷物中常见的DF成分AX的合生元组合中。