Toxicity of mine dump effluent containing five hexachlorocyclohexane (α, β, γ, δ and ε-HCH, sum 159.4 µg/L) and two trichlorobenzene (TCB, sum 65.2 µg/L) isomers to two microalgae (Scenedesmus quadricauda and Coccomyxa subellipsoidea) was studied over 24 h exposure and also with 2- and 10-fold diluted stock solution (i.e. 1x, 0.5x and 0.1x strength). Individual isomers revealed rather dose-dependent accumulation typically higher in Scenedesmus than in Coccomyxa (max. sum of HCH 14.99 µg/g DW with bioaccumulation factor 94) and δ-HCH was dominant isomer. TCB isomers showed low accumulation in algae. 0.1x dose elevated chlorophylls and carotenoids in Coccomyxa while enzymatic activities (SOD, CAT, and APX), thiols (glutathione and phytochelatin 2) and ascorbic acid were rather elevated by 1x dose in both species. Malic acid, rather than citric acid, increased in response to 0.5x and 1x concentration. Sum of fatty acids was higher in Coccomyxa than in Scenedesmus with palmitic, oleic, linoleic and α-linolenic acids being dominant compounds in both species. Detailed profiling revealed that saturated and monounsaturated fatty acids increased in Coccomyxa while polyunsaturated fatty acids in Scenedesmus in response to increasing dose of organochlorines. Accumulation of organochlorines and metabolic responses in algae are reported here for the first time.

含有五个六氯环己烷（α，β，γ，δ和ε-HCH，总计159.4 µg / L）和两个三氯苯（TCB，总计65.2 µg / L）异构体的矿山排泄物对两种微藻（Scendesmus quadricauda和Coccomyxa subellipsoidea）的毒性。在暴露24小时后，还用2倍和10倍稀释的原液（即1倍，0.5倍和0.1倍的强度）研究了SnO2。单个异构体的剂量依赖性积累通常在景天草中要高于球孢霉（HCH的最大总和为14.99 µg / g DW，具有生物积累因子94），而δ-HCH是主要的异构体。TCB异构体在藻类中显示低积累。球孢菌中叶绿素和类胡萝卜素的剂量增加0.1倍而酶活性（SOD，CAT和APX），硫醇（谷胱甘肽和植物螯合素2）和抗坏血酸在两个物种中的剂量都提高了1倍。苹果酸，而不是柠檬酸，响应于0.5倍和1倍浓度而增加。脂肪酸的总和是在更高的胶球藻比栅藻与棕榈酸，油酸，亚油酸和α亚麻酸酸是在这两个物种主导化合物。详细的评测表明，饱和的和单不饱和脂肪酸增加的胶球藻而多不饱和脂肪酸在栅藻响应于增加的有机氯剂量。首次报道了藻类中有机氯的积累和代谢反应。