As the worldwide application of silver nanomaterials in commercial products increases every year, and concern about the environmental risks of such nanoparticles also grows. A clear understanding of how different characteristics of nanoparticles contribute in their toxic behavior to organisms are imperative for predicting and control nanotoxicity. Within our research, we investigated the toxic effect of two types of silver nanoparticles (spherical and flat Ag nanoparticles) on zebrafish (Danio rerio) embryos. Particular interest was paid to proper characterization of Ag nanoparticles initially and during the experiment. A proper test medium was found and used for ecotoxicity evaluation. The behavior of flat silver nanoparticles with respect to embryos of zebrafish was analyzed and compared to the ecotoxicity of silver ionic form (AgNO₃). Both types of nanoparticles showed a more pronounced toxic effect to Danio rerio embryos than silver ions (AgNO₃), while silver nanoplates were more harmful than Ag nanospheres. While previous investigations showed that toxicity of Ag nanoparticles can be explained by the presence of Ag⁺ in solution of silver nanoparticles, our results demonstrate that the harmful effects of nanosilver may be associated with silver nanoparticles themselves than with ionic silver released into solution.

随着银纳米材料在商业产品中的全球应用逐年增加，对此类纳米颗粒的环境风险的关注也日益增加。明确了解纳米粒子的不同特性如何导致其对生物体的毒性行为，对于预测和控制纳米毒性至关重要。在我们的研究中，我们研究了两种类型的银纳米颗粒（球形和扁平Ag纳米颗粒）对斑马鱼（Danio rerio）胚胎的毒性作用。最初和实验期间，人们对银纳米颗粒的正确表征特别感兴趣。找到了合适的测试介质并将其用于生态毒性评估。分析了扁平银纳米颗粒相对于斑马鱼胚胎的行为，并将其与银离子形式（AgNO）的生态毒性进行了比较₃）。两种类型的纳米颗粒都对丹尼奥雷里奥胚胎表现出比银离子（AgNO ₃）更明显的毒性作用，而银纳米板比Ag纳米球更具危害性。尽管先前的研究表明，银纳米颗粒溶液中存在银⁺可以解释银纳米颗粒的毒性，但我们的结果表明，纳米银的有害作用可能与银纳米颗粒本身有关，而不是与释放到溶液中的离子银有关。