当前位置：
X-MOL 学术
›
Chem. Bio. Drug Des.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Identification and characterization of novel host defense peptides from the skin secretion of the fungoid frog, Hydrophylax bahuvistara (Anura: Ranidae).
Chemical Biology & Drug Design ( IF 3.2 ) Pub Date : 2017-02-11 , DOI: 10.1111/cbdd.12937 Thundi Parambil Vasanth Kumar Vineeth Kumar 1 , Radhamony Asha 2 , Gopal Shyla 1 , Sanil George 1
Chemical Biology & Drug Design ( IF 3.2 ) Pub Date : 2017-02-11 , DOI: 10.1111/cbdd.12937 Thundi Parambil Vasanth Kumar Vineeth Kumar 1 , Radhamony Asha 2 , Gopal Shyla 1 , Sanil George 1
Affiliation
|
Two novel peptides (brevinin1 HYba1 and brevinin1 HYba2) were identified from the skin secretion of the frog Hydrophylax bahuvistara, endemic to Western Ghats, India, and their amino acid sequences were confirmed using cDNA cloning and LC/MS/MS. Antibacterial, hemolytic, and cytotoxic activities of brevinin1 peptides and their synthetic analogs (amidated C-terminus) were investigated and compared. All the peptides except the acidic forms showed antibacterial activity against all tested Gram-positive and Gram-negative bacteria. They exhibited low hemolysis on human erythrocytes and showed potent cytotoxic activity against Hep 3B cancer cell line. Upon amidation, the peptides showed increased activity against the tested microbes without altering their hemolytic and cytotoxic properties. The study also emphasizes the need for screening endemic amphibian fauna of Western Ghats, as a potential source of host defense peptides with possible therapeutic applications in the future.
中文翻译：
鉴定和表征新颖的宿主防御肽，该真菌是从真菌青蛙蛙类嗜水菌（Hydrophylax bahuvistara，Anura：Ranidae）的皮肤分泌物中获得的。
从印度西高止山脉特有的蛙类嗜水菌的皮肤分泌物中鉴定出两个新颖的肽（brevinin1 HYba1和brevinin1 HYba2），并使用cDNA克隆和LC / MS / MS确认了它们的氨基酸序列。研究并比较了brevinin1肽及其合成类似物（酰胺化的C端）的抗菌，溶血和细胞毒性活性。除酸性形式外，所有肽均对所有测试的革兰氏阳性和革兰氏阴性细菌均具有抗菌活性。他们在人类红细胞上显示出低溶血性，并显示出对Hep 3B癌细胞系的有效细胞毒活性。酰胺化后，这些肽对被测微生物表现出增强的活性，而不会改变其溶血和细胞毒性特性。
更新日期：2017-02-11
中文翻译：
鉴定和表征新颖的宿主防御肽，该真菌是从真菌青蛙蛙类嗜水菌（Hydrophylax bahuvistara，Anura：Ranidae）的皮肤分泌物中获得的。
从印度西高止山脉特有的蛙类嗜水菌的皮肤分泌物中鉴定出两个新颖的肽（brevinin1 HYba1和brevinin1 HYba2），并使用cDNA克隆和LC / MS / MS确认了它们的氨基酸序列。研究并比较了brevinin1肽及其合成类似物（酰胺化的C端）的抗菌，溶血和细胞毒性活性。除酸性形式外，所有肽均对所有测试的革兰氏阳性和革兰氏阴性细菌均具有抗菌活性。他们在人类红细胞上显示出低溶血性，并显示出对Hep 3B癌细胞系的有效细胞毒活性。酰胺化后，这些肽对被测微生物表现出增强的活性，而不会改变其溶血和细胞毒性特性。
京公网安备 11010802027423号