Heterologous and High Production of Ergothioneine in Escherichia coli,Journal of Agricultural and Food Chemistry

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Heterologous and High Production of Ergothioneine in Escherichia coli
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-15 00:00:00 , DOI: 10.1021/acs.jafc.7b04924
Ryo Osawa , Tomoyuki Kamide , Yasuharu Satoh , Yusuke Kawano ₁ , Iwao Ohtsu ₁ , Tohru Dairi

Affiliation

Ergothioneine (ERG) is a histidine-derived thiol compound suggested to function as an antioxidant and cytoprotectant in humans. Therefore, experimental trials have been conducted applying ERG from mushrooms in dietary supplements and as a cosmetic additive. However, this method of producing ERG is expensive; therefore, alternative methods for ERG supply are required. Five Mycobacterium smegmatis genes, egtABCDE, have been confirmed to be responsible for ERG biosynthesis. This enabled us to develop practical fermentative ERG production by microorganisms. In this study, we carried out heterologous and high-level production of ERG in Escherichia coli using the egt genes from M. smegmatis. By high production of each of the Egt enzymes and elimination of bottlenecks in the substrate supply, we succeeded in constructing a production system that yielded 24 mg/L (104 μM) secreted ERG.

中文翻译：

大肠杆菌中的异源和高产量的麦角硫因

麦角硫氨酸（ERG）是一种组氨酸衍生的硫醇化合物，被认为在人类中起着抗氧化剂和细胞保护剂的作用。因此，已经进行了实验性试验，将蘑菇中的ERG用作膳食补充剂并作为化妆品添加剂。但是，这种生产ERG的方法很昂贵。因此，需要替代的方法来供应ERG。已经证实有五个耻垢分枝杆菌基因egtABCDE负责ERG的生物合成。这使我们能够通过微生物开发实际的发酵ERG生产。在这项研究中，我们使用耻垢分枝杆菌的egt基因在大肠杆菌中进行了异源和高水平的ERG生产。通过大量生产每种Egt酶并消除底物供应中的瓶颈，我们成功构建了一个生产系统，该系统可产生24 mg / L（104μM）分泌的ERG。

更新日期：2018-01-15

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