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Accelerated Development of Hippocampal Neurons and Limited Adhesion of Astrocytes on Negatively Charged Surfaces
Langmuir ( IF 3.7 ) Pub Date : 2018-01-09 00:00:00 , DOI: 10.1021/acs.langmuir.7b03132 Mi-Hee Kim 1 , Ji Hun Park 1 , Sunghoon Joo 1 , Daewha Hong 2 , Matthew Park 1 , Ji Yu Choi 1 , Hye Won Moon 1 , Yang-Gyun Kim 3 , Kyungtae Kang 4 , Insung S. Choi 1
Langmuir ( IF 3.7 ) Pub Date : 2018-01-09 00:00:00 , DOI: 10.1021/acs.langmuir.7b03132 Mi-Hee Kim 1 , Ji Hun Park 1 , Sunghoon Joo 1 , Daewha Hong 2 , Matthew Park 1 , Ji Yu Choi 1 , Hye Won Moon 1 , Yang-Gyun Kim 3 , Kyungtae Kang 4 , Insung S. Choi 1
Affiliation
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This work examines the development of primary neurons and astrocytes on thoroughly controlled functional groups. Negatively charged surfaces presenting carboxylate (COO–) or sulfonate (SO3–) groups prove beneficial to neuronal behavior, in spite of their supposed repulsive electrostatic interactions with cellular membranes. The adhesion and survival of primary hippocampal neurons on negatively charged surfaces are comparable to or slightly better than those on positively charged (poly-d-lysine-coated) surfaces, and neuritogenesis and neurite outgrowth are accelerated on COO– and SO3– surfaces. Moreover, such favorable influences of the negatively charged surfaces are only seen in neurons but not for astrocytes. Our results indicate that the in vitro developmental behavior of primary hippocampal neurons is sophisticatedly modulated by angstrom-sized differences in chemical structure or the charge density of the surface. We believe that this work provides new implications for understanding neuron–material interfaces as well as for establishing new ways to fabricate neuro-active surfaces.
中文翻译：
海马神经元的加速发展和负电荷表面上星形胶质细胞的有限粘附。
这项工作检查了在完全受控的功能基团上原代神经元和星形胶质细胞的发育。带有羧酸根（COO –）或磺酸根（SO 3 –）基团的带负电荷的表面尽管对细胞膜具有排斥性静电相互作用，但仍被证明对神经元行为有益。上负电荷的表面的密合性和原代海马神经元的存活是相当或略好于那些在带正电荷的（聚d赖氨酸包被的）表面，和神经突和神经突增生是在加速COO -和SO 3 -表面。此外，带负电荷的表面的这种有利影响仅在神经元中可见，而对于星形胶质细胞则没有。我们的结果表明，原位海马神经元的体外发育行为受到化学结构或表面电荷密度的埃大小差异的精确控制。我们认为，这项工作为理解神经元-材料界面以及建立新的制造神经活性表面的方法提供了新的含义。
更新日期：2018-01-09
中文翻译：
海马神经元的加速发展和负电荷表面上星形胶质细胞的有限粘附。
这项工作检查了在完全受控的功能基团上原代神经元和星形胶质细胞的发育。带有羧酸根（COO –）或磺酸根（SO 3 –）基团的带负电荷的表面尽管对细胞膜具有排斥性静电相互作用，但仍被证明对神经元行为有益。上负电荷的表面的密合性和原代海马神经元的存活是相当或略好于那些在带正电荷的（聚d赖氨酸包被的）表面，和神经突和神经突增生是在加速COO -和SO 3 -表面。此外，带负电荷的表面的这种有利影响仅在神经元中可见，而对于星形胶质细胞则没有。我们的结果表明，原位海马神经元的体外发育行为受到化学结构或表面电荷密度的埃大小差异的精确控制。我们认为，这项工作为理解神经元-材料界面以及建立新的制造神经活性表面的方法提供了新的含义。
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