Study of Fluorescence Quenching Ability of Graphene Oxide with a Layer of Rigid and Tunable Silica Spacer,Langmuir

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Study of Fluorescence Quenching Ability of Graphene Oxide with a Layer of Rigid and Tunable Silica Spacer
Langmuir ( IF 3.7 ) Pub Date : 2018-01-05 00:00:00 , DOI: 10.1021/acs.langmuir.7b03465
Xu Wu ₁ , Yuqian Xing ₁ , Kevin Zeng ₁ , Kirby Huber ₁ , Julia Xiaojun Zhao ₁

Affiliation

The fluorescence quenching property of graphene oxide (GO) has been newly demonstrated and applied for fluorescence imaging and biosensing. In this work, a new nanostructure was designed for effectively studying the quenching ability of GO. The key element in this design is the fabrication of a layer of rigid and thickness adjustable silica spacer for manipulating the distance between the GO and fluorophores. First, a silica core modified with organic dye molecules was prepared, followed by the formation of a silica shell with a tunable thickness. Afterward, the GO was wrapped around silica nanoparticles based on the electrostatic interaction between the negatively charged GO and positively charged silica. The quenching efficiency of GO to different dye molecules was studied at various spacer thicknesses and varying concentrations of GO. Fluorescence lifetime of fluorophores was measured to determine the quenching mechanism. We found that the quenching efficiency of GO was still around 30% when the distance between dyes and GO was increased to more than 30 nm, which indicated the long-distance quenching ability of GO and confirmed the previous theoretical calculation. The quenching mechanisms were proposed schematically based on our experimental results. We expected that the proposed nanostructure could act as a feasible model for studying GO quenching property and shed light on designing GO-based fluorescence sensing systems.

中文翻译：

刚性可调节二氧化硅隔层氧化石墨烯的荧光猝灭能力研究

氧化石墨烯（GO）的荧光猝灭特性已经得到了新的证明，并已用于荧光成像和生物传感。在这项工作中，设计了一种新的纳米结构，以有效地研究GO的淬灭能力。此设计的关键要素是制造一层刚性且厚度可调的二氧化硅垫片，以控制GO和荧光团之间的距离。首先，制备用有机染料分子改性的二氧化硅核，然后形成具有可调厚度的二氧化硅壳。然后，基于带负电的GO和带正电的二氧化硅之间的静电相互作用，将GO包裹在二氧化硅纳米颗粒周围。研究了GO在不同间隔层厚度和GO浓度下对不同染料分子的猝灭效率。测量荧光团的荧光寿命以确定猝灭机理。我们发现，当染料与GO之间的距离增加到30 nm以上时，GO的猝灭效率仍在30％左右，这表明GO的长距离猝灭能力并证实了先前的理论计算。根据我们的实验结果，示意性地提出了淬灭机理。我们期望提出的纳米结构可以作为研究GO猝灭特性的可行模型，并为设计基于GO的荧光传感系统提供参考。这表明了GO的长距离淬火能力，并证实了先前的理论计算。根据我们的实验结果，示意性地提出了淬灭机理。我们期望提出的纳米结构可以作为研究GO猝灭特性的可行模型，并为设计基于GO的荧光传感系统提供参考。这表明了GO的长距离淬火能力，并证实了先前的理论计算。根据我们的实验结果，示意性地提出了淬灭机理。我们期望提出的纳米结构可以作为研究GO猝灭特性的可行模型，并为设计基于GO的荧光传感系统提供参考。

更新日期：2018-01-05

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