Spectral (optical absorption and emission) properties of three amphoteric phosphorous(V)-phthalocyanine derivatives, [P(Pc)(O)OH], where Pc = tetra(tert-butyl)phthalocyaninate (tbpc), tetrakis(2′,6′-dimethylphenoxy)phthalocyaninate (tppc), and octakis(4′-tert-butylphenoxy)phthalocyaninate (obppc), have been investigated in ethanolic solutions. Spectral changes upon protonation/deprotonation (the reaction sites have been determined to be their axial ligands by magnetic circular dichroism study) are drastic and rapid. All the initial ([P(Pc)(O)OH]), protonated ([P(Pc)(OH)₂]⁺), and deprotonated ([P(Pc)(O)₂]⁻) species are possessed with sufficient brightness (defined as the product of their molar extinction coefficient, ε (in M^− 1 cm^− 1), and fluorescence quantum yield, Φ_F) in bio-imaging window (650–900 nm). For example, spectral characteristics of the tbpc derivatives have been determined as follows: ε = 1.65 × 10⁵ (absorption maximum 676 nm) and Φ_F = 0.80 (emission maximum 686 nm) for [P(tbpc)(O)(OH)] while ε = 1.45 × 10⁵ (697 nm) and Φ_F = 0.27 (714 nm) for [P(tbpc)(OH)₂]⁺, and ε = 2.25 × 10⁵ (662 nm) and Φ_F = 0.90 (667 nm) for [P(tbpc)(O)₂]⁻. Emission of tppc and obppc derivatives behave in essentially the same manner irrespective of nature of the peripheral substituents and hence Φ_F values are greater with increasing emission peak wavenumbers in line with the “energy gap law”. These characteristics make these compounds promising candidates as chemical probes for deep-tissue bio-imaging.

三种两性磷（V）-酞菁衍生物[P（Pc）（O）OH]的光谱（光学吸收和发射）性质，其中Pc =酞菁四（叔丁基）酞菁（tbpc），四（2'，6已经在乙醇溶液中研究了'-二甲基苯氧基）酞菁盐（tppc）和辛基（4'-叔丁基苯氧基）酞菁盐（obppc）。质子化/去质子化时的光谱变化是剧烈且快速的（反应位点已通过磁性圆二色性研究确定为它们的轴向配体）。所有初始（[P（Pc）（O）OH]），质子化（[P（Pc）（OH）₂ ] ⁺）和去质子化（[P（Pc）（O）₂ ] ^-）物种具有以足够的亮度（被定义为它们的摩尔消光系数，ε（在M的产物^{- 1} 厘米^{1 -} ），和荧光量子产率，Φ _˚F在生物成像窗口）（650-900纳米）。例如，TBPC衍生物的光谱特性已经被确定为如下：ε= 1.65×10 ⁵（最大吸收676纳米）和Φ _˚F  = 0.80（最大发射686纳米）为[P（TBPC）（O）（OH） ]，而ε= 1.45×10 ⁵（697纳米）和Φ _˚F  = 0.27（714纳米）为[P（TBPC）（OH）₂ ] ⁺，和ε= 2.25×10 ⁵（662纳米）和Φ _˚F  = 0.90对于[P（tbpc）（O）₂为（667 nm）] ^-。TPPC和obppc衍生物的发射表现在基本相同的方式而不管周围取代基，因此Φ的性质的_˚F值是较大值与“能隙法”在线增加发射峰的波数。这些特性使这些化合物有望成为深组织生物成像的化学探针。