Chronoamperometry-Based Redox Cycling for Application to Immunoassays,ACS Sensors

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Chronoamperometry-Based Redox Cycling for Application to Immunoassays
ACS Sensors ( IF 8.2 ) Pub Date : 2018-01-11 00:00:00 , DOI: 10.1021/acssensors.7b00681
Ga-Yeon Lee ₁ , Jun-Hee Park ₁ , Young Wook Chang ₁ , Sungbo Cho ₂ , Min-Jung Kang ₃ , Jae-Chul Pyun ₁

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In this work, the chronoamperometry-based redox cycling of 3,3′,5,5′-tetramethylbenzidine (TMB) was performed by using interdigitated electrode (IDE). The signal was obtained from two sequential chronoamperometric profiles: (1) with the generator at the oxidative potential of TMB and the collector at the reductive potential of TMB, and (2) with the generator at the reductive potential of TMB and the collector at the oxidative potential of TMB. The chronoamperometry-based redox cycling (dual mode) showed a sensitivity of 1.49 μA/OD, and the redox cycling efficiency was estimated to be 94% (n = 10). The sensitivities of conventional redox cycling with the same interdigitated electrode and chronoamperometry using a single working electrode (single mode) were estimated to be 0.67 μA/OD and 0.18 μA/OD, respectively. These results showed that the chronoamperometry-based redox cycling (dual mode) could be more effectively used to quantify the oxidized TMB than other amperometric methods. The chronoamperometry-based redox cycling (dual mode) was applied to immunoassays using a commercial ELISA kit for medical diagnosis of the human hepatitis B virus surface antigen (hHBsAg). Finally, the chronoamperometry-based redox cycling (dual mode) provided more than a 10-fold higher sensitivity than conventional chronoamperometry using a single working electrode (single mode) when applied to a commercial ELISA kit for medical diagnosis of hHBsAg.

中文翻译：

基于计时电流法的氧化还原循环在免疫分析中的应用

在这项工作中，通过使用叉指电极（IDE）进行了基于计时电流法的3,3'，5,5'-四甲基联苯胺（TMB）的氧化还原循环。该信号是从两个连续的计时电流曲线获得的：（1）发生器处于TMB的氧化电位，集电极处于TMB的还原电位，（2）发生器处于TMB的还原电位，而集电极处于TMB的还原电位。 TMB的氧化电位。基于计时电流法的氧化还原循环（双模式）显示出1.49μA/ OD的灵敏度，并且氧化还原循环效率估计为94％（n= 10）。使用相同的叉指电极和使用单个工作电极（单模）的计时电流法进行的传统氧化还原循环的灵敏度分别估计为0.67μA/ OD和0.18μA/ OD。这些结果表明，与其他安培法相比，基于计时安培的氧化还原循环（双模式）可以更有效地用于量化氧化的TMB。使用商用ELISA试剂盒将基于计时电流法的氧化还原循环（双模式）应用于免疫测定，以对人类乙型肝炎病毒表面抗原（hHBsAg）进行医学诊断。最终，基于计时电流法的氧化还原循环（双模式）比使用单个工作电极（单模式）的传统计时电流法提供的灵敏度高10倍以上，应用于商业ELISA试剂盒用于hHBsAg的医学诊断。

更新日期：2018-01-11

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