New Frontiers and Challenges for Single-Cell Electrochemical Analysis,ACS Sensors

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New Frontiers and Challenges for Single-Cell Electrochemical Analysis
ACS Sensors ( IF 8.2 ) Pub Date : 2018-01-10 00:00:00 , DOI: 10.1021/acssensors.7b00711
Jingjing Zhang ₁ , Junyu Zhou ₁ , Rongrong Pan ₁ , Dechen Jiang ₁ , James D. Burgess ₂ , Hong-Yuan Chen ₁

Affiliation

Previous measurements of cell populations might obscure many important cellular differences, and new strategies for single-cell analyses are urgently needed to re-examine these fundamental biological principles for better diagnosis and treatment of diseases. Electrochemistry is a robust technique for the analysis of single living cells that has the advantages of minor interruption of cellular activity and provides the capability of high spatiotemporal resolution. The achievements of the past 30 years have revealed significant information about the exocytotic events of single cells to elucidate the mechanisms of cellular activity. Currently, the rapid developments of micro/nanofabrication and optoelectronic technologies drive the development of multifunctional electrodes and novel electrochemical approaches with higher resolution for single cells. In this Perspective, three new frontiers in this field, namely, electrochemical microscopy, intracellular analysis, and single-cell analysis in a biological system (i.e., neocortex and retina), are reviewed. The unique features and remaining challenges of these techniques are discussed.

中文翻译：

单细胞电化学分析的新领域和新挑战

先前对细胞群体的测量可能掩盖了许多重要的细胞差异，因此迫切需要用于单细胞分析的新策略来重新检查这些基本生物学原理，以更好地诊断和治疗疾病。电化学是一种用于分析单个活细胞的强大技术，其优点是细胞活性受到轻微干扰，并具有高时空分辨率。过去30年的成就揭示了有关单细胞胞吐事件的重要信息，以阐明细胞活性的机制。当前，微/纳米制造和光电技术的飞速发展推动了多功能电极的发展，并为单电池提供了更高分辨率的新型电化学方法。在此观点中，回顾了该领域的三个新领域，即电化学显微镜，细胞内分析和生物系统（即新皮层和视网膜）中的单细胞分析。讨论了这些技术的独特功能和尚存的挑战。

更新日期：2018-01-10

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