Stabilization of the Reductase Domain in the Catalytically Self‐Sufficient Cytochrome P450BM3 by Consensus‐Guided Mutagenesis,ChemBioChem

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Stabilization of the Reductase Domain in the Catalytically Self‐Sufficient Cytochrome P450BM3 by Consensus‐Guided Mutagenesis
ChemBioChem ( IF 2.6 ) Pub Date : 2018-02-12 , DOI: 10.1002/cbic.201700546
Gloria Saab-Rincón ₁ , Hanan Alwaseem ₂ , Valeria Guzmán-Luna ₁ , Leticia Olvera ₁ , Rudi Fasan ₂

Affiliation

Handling the heat: Consensus‐guided mutagenesis was applied to stabilize the reductase domain of the biotechnologically useful P450_BM3 enzyme. The evolved variants exhibited improved robustness to thermal denaturation and increased catalytic performance at elevated temperatures.