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Stabilization of the Reductase Domain in the Catalytically Self‐Sufficient Cytochrome P450BM3 by Consensus‐Guided Mutagenesis
ChemBioChem ( IF 3.2 ) Pub Date : 2018-02-12 , DOI: 10.1002/cbic.201700546
Gloria Saab-Rincón 1 , Hanan Alwaseem 2 , Valeria Guzmán-Luna 1 , Leticia Olvera 1 , Rudi Fasan 2
Affiliation  

Handling the heat: Consensus‐guided mutagenesis was applied to stabilize the reductase domain of the biotechnologically useful P450BM3 enzyme. The evolved variants exhibited improved robustness to thermal denaturation and increased catalytic performance at elevated temperatures.
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中文翻译:

共识指导诱变稳定自催化细胞色素P450BM3中的还原酶结构域。

处理热量:应用共识性诱变技术来稳定生物技术上有用的P450 BM3酶的还原酶结构域。进化的变体表现出对热变性的改善的鲁棒性和在升高的温度下增加的催化性能。
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更新日期:2018-02-12
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