Abstract Herein, a new and facile approach by in situ cross-linking polymerizationcoupled with a post-functionalization procedure was developed to prepare functional PES composite membranes to improve its over-all performance as hemodialysis membrane. In our strategy, poly(acrylate acid-co-2-hydroxyethyl methacrylate) P(AA-co-HEMA) as a macromonomer, was firstly synthesized by free radical copolymerization, then blended in PES solution and in situ cross-linked polymerization via the reaction of the hydroxy group of HEMA and the isocyanate group of 4,4′-diphenylmethane diisocyanate (MDI) to prepare the carboxylic membranes. To further enhance the hemocompatibility, the carboxylic membranes were post-functionalized by grafting sulfonic acid groups onto the membrane surfaces via a “grafting from” method. The chemical compositions and surface morphologies of the prepared membranes were investigated by ATR-FTIR, XPS, SEM and AFM. After introducing the functional groups, the surface hydrophilicity of the membranes was significantly improved, demonstrated by the reduced water contact angles (WCAs) and enhanced permeability. The decreased protein adsorptions and higher flux recovery ratios (FRRs) revealed that the modified membranes showed improved protein antifouling property. The improvement of hemocompatibility was confirmed by the inhibited platelet adhesion, prolonged clotting times, and suppressed blood-related complement activation. Moreover, the post-functionalized membranes also showed good cytocompatibility. In general, the in situ cross-linking technique is a suitable route in the field of membrane modification for its facile and easy operation; and the post-functionalization operation could endow the membranes with efficiently improved properties.

中文翻译：

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羧基聚醚砜复合膜的后功能化

摘要 在此，开发了一种新的、简便的方法，通过原位交联聚合结合后功能化程序来制备功能性 PES 复合膜，以提高其作为血液透析膜的整体性能。在我们的策略中，聚（丙烯酸-co-2-羟乙基甲基丙烯酸酯）P（AA-co-HEMA）作为大分子单体，首先通过自由基共聚反应合成，然后与 PES 溶液共混并通过原位交联聚合HEMA的羟基与4,4'-二苯基甲烷二异氰酸酯（MDI）的异氰酸酯基反应制备羧酸膜。为了进一步提高血液相容性，通过“接枝”方法将磺酸基团接枝到膜表面，对羧酸膜进行后功能化。通过 ATR-FTIR、XPS、SEM 和 AFM 研究了制备的膜的化学成分和表面形貌。引入官能团后，膜的表面亲水性得到显着改善，这表现为水接触角 (WCA) 降低和渗透性增强。减少的蛋白质吸附和更高的通量回收率 (FRR) 表明改性膜显示出改善的蛋白质防污性能。通过抑制血小板粘附、延长凝血时间和抑制血液相关补体激活，证实了血液相容性的改善。此外，后功能化膜也表现出良好的细胞相容性。一般来说，原位交联技术简便易行，是膜改性领域的一条适宜途径。后功能化操作可以赋予膜有效改善的性能。