Positively charged polyhexamethylene guanidine hydrochloride-stabilized silver nanoparticles (PHMG-AgNPs) were prepared and applied as a colorimetric probe for single-step determination of pyrophosphate and sulfate. The approach is based on the nanoparticles aggregation leading to change in their absorption spectra and color of the solution. Due to both electrostatic and steric stabilization these nanoparticles show decreased sensitivity relatively to many common anions, which allows for simple and rapid direct single-step determination of pyrophosphate and sulfate. Effects of different factors (time of interaction, pH, concentrations of anions and the nanoparticles) on aggregation of PHMG-AgNPs and analytical performance of the procedure were investigated. The method allows for the determination of pyrophosphate and sulfate in the range of 0.16–2 μg mL⁻¹ and 20–80 μg mL⁻¹ with RSD of 2–5%, respectively. The analysis can be performed using either spectrophotometry or naked-eye detection. Practical application of the method was shown by the example of pyrophosphate determination in baking powder sample.

制备了带正电荷的聚六亚甲基胍盐酸盐稳定的银纳米颗粒（PHMG-AgNPs），并将其用作比色探针，用于一步测定焦磷酸盐和硫酸盐。该方法基于纳米颗粒聚集，从而导致其吸收光谱和溶液颜色发生变化。由于静电和位阻稳定，这些纳米颗粒相对于许多常见阴离子显示出降低的灵敏度，这使得焦磷酸和硫酸根的简单，快速直接一步测定成为可能。研究了不同因素（相互作用时间，pH，阴离子和纳米颗粒的浓度）对PHMG-AgNPs聚集的影响以及该方法的分析性能。该方法可以测定0范围内的焦磷酸盐和硫酸盐。^-1和20–80μgmL ^-1，RSD分别为2–5％。可以使用分光光度法或肉眼检测进行分析。以发酵粉样品中焦磷酸盐的测定为例说明了该方法的实际应用。