Abstract To improve the cell suspension of the gelatin-methacrylate(GelMA) bioink, the viscosity of the ink solution was controlled by biocompatible silk fibroin(SF) particles. The viscosity of the GelMA solution increased about 1.4 × 102 folds by the addition of 1 w/v% SF particles. The increase of solution viscosity reduced the displacement of cells and retarded the cell sedimentation significantly. SF-GelMA bioink containing 1 w/v% SF was non-cytotoxic and used for the 3D bioprinting by directly projecting a feature onto the bioink. The cells encapsulated in the structures showed a high level of metabolic activity and demonstrated that SF-GelMA was a biocompatible material.

中文翻译：

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溶液粘度对甲基丙烯酸明胶/丝素蛋白生物墨水 DLP 3D 打印中细胞沉降延迟的影响

摘要 为改善甲基丙烯酸明胶（GelMA）生物墨水的细胞悬浮性，通过生物相容性丝素蛋白（SF）颗粒控制墨水溶液的粘度。通过添加 1 w/v% SF 颗粒，GelMA 溶液的粘度增加了约 1.4 × 102 倍。溶液粘度的增加减少了细胞的位移并显着延缓了细胞沉降。含有 1 w/v% SF 的 SF-GelMA 生物墨水无细胞毒性，可通过将特征直接投影到生物墨水上来用于 3D 生物打印。封装在结构中的细胞显示出高水平的代谢活性，并证明 SF-GelMA 是一种生物相容性材料。