Purification and Characterization of Peptides Inhibiting MMP-1 Activity with C Terminate of Gly-Leu from Simulated Gastrointestinal Digestion Hydrolysates of Tilapia (Oreochromis niloticus) Skin Gelatin,Journal of Agricultural and Food Chemistry

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Purification and Characterization of Peptides Inhibiting MMP-1 Activity with C Terminate of Gly-Leu from Simulated Gastrointestinal Digestion Hydrolysates of Tilapia (Oreochromis niloticus) Skin Gelatin
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-10 00:00:00 , DOI: 10.1021/acs.jafc.7b04196
Sun Liping ₁ , Liu Qiuming ₁ , Fan Jian ₁ , Li Xiao ₁ , Zhuang Yongliang ₁

Affiliation

Tilapia skin gelatin hydrolysates (TSGHs) were prepared by simulated gastrointestinal digestion and separated by gel filtration and semi-preparative reversed-phase high-performance liquid chromatography. The anti-photoaging effects were evaluated using an ultraviolet radiation B (UVB)-induced mouse embryonic fibroblast (MEF) photoaging model in vitro. Three fractions from TSGHs with high inhibitory intercellular matrix metalloproteinase-1 (MMP-1) activities and reactive oxygen species (ROS) production were obtained. Three key peptides, GYTGL, LGATGL, and VLGL, were identified, and their C terminate was Gly-Leu. Three peptides were synthesized and exhibited a significant inhibition of intercellular MMP-1 activity and ROS production. Furthermore, three peptides inhibiting MMP-1 activities were evaluated through their docking of S₁′ and S₃′ active pockets of MMP-1. Hydrogen bonds and C terminate Gly-Leu played important roles. Finally, the protective effects of three peptides on intercellular collagen in UVB-induced MEFs were compared. Our results indicated that tilapia gelatin peptides exhibited potential activities to prevent and regulate photoaging.

中文翻译：

纯化和肽MMP-1抑制活性的表征C来自罗非鱼的模拟胃肠消化水解产物（甘氨酸-亮氨酸的终止罗非鱼）皮肤明胶

罗非鱼皮肤明胶水解物（TSGHs）是通过模拟胃肠消化法制备的，并通过凝胶过滤和半制备反相高效液相色谱法进行分离。在体外使用紫外线B（UVB）诱导的小鼠胚胎成纤维细胞（MEF）光老化模型评估其抗光老化作用。从具有高抑制性细胞间基质金属蛋白酶-1（MMP-1）活性和活性氧（ROS）产生的TSGH中获得了三个馏分。鉴定了三个关键肽GYTGL，LGATGL和VLGL，其C末端为Gly-Leu。合成了三种肽，并显示出对细胞间MMP-1活性和ROS产生的显着抑制作用。此外，通过S ₁ '和S _3的对接评估了三种抑制MMP-1活性的肽。MMP-1的活性口袋。氢键和C终止Gly-Leu发挥了重要作用。最后，比较了三种肽对UVB诱导的MEF中细胞间胶原的保护作用。我们的结果表明，罗非鱼明胶肽具有潜在的预防和调节光老化的活性。

更新日期：2018-01-10

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