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Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1
Journal of Functional Foods ( IF 3.8 ) Pub Date : 2017-12-22 , DOI: 10.1016/j.jff.2017.12.039
Chaoran Liu , Man Wing Choi , Xiaojie Li , Peter C.K. Cheung

Two high molecular weight (MW larger than 788 kDa) mushroom polysaccharides extracted by hot water (PRW) and micronized sonication-assisted cold alkali (mPRSon), were isolated from the sclerotia of Polyporus rhinocerus. PRW was a polysaccharide-protein complex with a highly branched (degree of branching DB 0.60) β-D-mannoglucan while mPRSon was a hyper-branched β-glucan (DB 0.85). PRW could bind to complement receptor 3 (CR3) and significantly induced THP-1 differentiation evidenced by cell morphology and enhanced phagocytic activity. A significant increase in the expression of surface markers including CD14, CD11b and HLA-DR as well as enhanced production of cytokines including IL-8, RANTES, GRO, MCP-1, MCP-2 and IL-6 were observed on THP-1 cells treated with PRW. In contrast, mPRSon could not either activate the Dectin-1 signaling or induce the THP-1 differentiation. High MW and DB mushroom sclerotial polysaccharide which has a structure of heteroglycan and protein is more immunopotent than pure homoglycan.



中文翻译:

鼻菌中分离到的结构特征性蘑菇硬化多糖对人单核细胞THP-1的免疫调节作用

两个高的分子量(MW大于788 kDa)的通过热水(PRW)萃取和微粉化蘑菇多糖超声辅助的冷碱(mPRSon),购自的菌核分离猪苓rhinocerus。PRW是具有高度分支(分支度DB 0.60)的β-D-甘露聚糖的多糖-蛋白质复合物,而mPRSon是高度分支的β-葡聚糖(DB 0.85)。PRW可以结合补体受体3(CR3),并显着诱导THP-1分化,这由细胞形态和增强的吞噬活性证明。在THP-1上观察到表面标志物(包括CD14,CD11b和HLA-DR)的表达显着增加,并且细胞因子(包括IL-8,RANTES,GRO,MCP-1,MCP-2和IL-6)的产生增加PRW处理过的细胞。相反,mPRSon既不能激活Dectin-1信号传导,也不能诱导THP-1分化。具有杂聚糖和蛋白质结构的高分子量和DB蘑菇硬化多糖比纯高聚糖具有更高的免疫力。

更新日期:2017-12-22
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