The dyestuffs used in the textile sector are among the major pollutants and present as a polluting agent in the waters poured to the environment. Therefore, synthetic dyes led to the serious environmental pollution and threats to human health. The process of color removal from waste water of the textile industry is gaining importance from ecological point of view. Nowadays, the removal of dye materials is carried out by physical and chemical methods. However, the high cost of these methods and the resulting large amount of concentrated slurry causes problems. In recent years, it has been shown that white rot fungi have the ability to reduce the synthetic dye materials with their extracellular ligninolytic enzymes. Fungal biosorption is an economical, easy and efficient method for removing organic contaminants from aqueous media. In this study; the white rot fungus M. esculenta was immobilized into five different support materials (gelatin, polyurethane, kaolin, cellulose) and the removal of Reactive Orange 16 dye with these immobilized cells was investigated and compared. The best biological degradation of the dye was obtained by immobilization into polyurethane support material with M. esculenta (dye concentration: 10 mgL⁻¹) 90,38%. Samples were analyzed in FT-IR to determine possible metabolites that can be occurred as a result of biodegradation. The M. esculenta laccase, which may be responsible for biodegradation, was partially purified and identified by SDS-PAGE.

纺织部门使用的染料是主要污染物之一，并在倒入环境的水域中以污染剂的形式存在。因此，合成染料导致严重的环境污染和对人类健康的威胁。从生态学的角度来看，纺织工业废水的脱色过程正变得越来越重要。如今，染料的去除是通过物理和化学方法进行的。然而，这些方法的高成本以及所产生的大量浓缩浆液引起了问题。近年来，已经显示出白腐真菌具有利用其细胞外木质素分解酶还原合成染料材料的能力。真菌生物吸附是一种从水性介质中去除有机污染物的经济，简便且有效的方法。在这项研究中;esculenta菌被固定在五种不同的载体材料（明胶，聚氨酯，高岭土，纤维素）中，并研究了固定化细胞对活性橙16染料的去除情况并进行了比较。染料的最佳生物降解性是通过将固定化至具有esculenta的M. esculenta（染料浓度：10 mgL ^-1）为90.38％的聚氨酯载体材料获得的。在FT-IR中分析样品，以确定可能由于生物降解而发生的代谢产物。可能负责生物降解的M. esculenta漆酶已部分纯化，并通过SDS-PAGE鉴定。