Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Fabrication and assessment of an electrospun polymeric microfiber-based platform under bulk flow conditions with rapid and efficient antigen capture†
Analyst ( IF 3.6 ) Pub Date : 2017-12-22 00:00:00 , DOI: 10.1039/c7an01366c Carlton F. O. Hoy 1, 2, 3, 4, 5 , Keiichiro Kushiro 1, 2, 3, 4, 5 , Madoka Takai 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2017-12-22 00:00:00 , DOI: 10.1039/c7an01366c Carlton F. O. Hoy 1, 2, 3, 4, 5 , Keiichiro Kushiro 1, 2, 3, 4, 5 , Madoka Takai 1, 2, 3, 4, 5
Affiliation
|
This study investigated the fabrication and proof of concept design demonstrating rapid and highly sensitive antigen capture utilizing electrospun polystyrene (PS) microfiber mat substrates paired with vacuum pump pressurization to induce bulk flow. In comparison with conventional flat PS surfaces used for immunoassay purposes, this system optimizes the increased surface area of the electrospun polystyrene (ESPS) fiber mat substrates and the accelerated propagation of the antigen through the detection platform by using a vacuum pump to enable efficient and rapid antigen capture. The novelty of this work was demonstrated through a parametric study detailing how a fiber substrate can capture antigen sensitively and at high speeds. In terms of sensitivity, the current system is comparable to the conventionally used flat PS substrates. Additionally, the amount of antigen captured on a flat PS substrate in 60 minutes was surpassed in under 5 seconds when utilizing the ESPS-vacuum system. Three-dimensional ESPS fiber mats were then noted as a comparison between Damkohler numbers and between flat PS and ESPS-vacuum systems. The bulk flow of the ESPS-vacuum system allows for a Damkohler number of 0.37 indicating a balance between the flow rate and the reaction rate as opposed to a PS flat platform of 5.80 × 104 which illustrates a diffusion rate limited system. Finally, the overall ESPS-vacuum system was tested for its immunoassay capability. A sandwich fluorescence-based immunoassay was performed on both PS flat-diffusion and ESPS-vacuum systems. The ESPS-vacuum system indicated a wider detection range capability from 5 to 1000 ng mL−1 in comparison with the PS flat-diffusion system at 5 to 100 ng mL−1.
中文翻译：
在大流量条件下快速有效地捕获抗原的静电纺基于聚合物超细纤维的平台的制造和评估†
这项研究调查了设计和概念设计的证明，这些演示证明了利用电纺聚苯乙烯（PS）超细纤维毡基质与真空泵加压配对以诱导大流量快速而高度敏感的抗原捕获。与用于免疫测定的常规平坦PS表面相比，该系统通过使用真空泵优化了电纺聚苯乙烯（ESPS）纤维垫基质的增加的表面积以及抗原通过检测平台的加速传播，从而实现了高效，快速抗原捕获。通过一项参数研究证明了这项工作的新颖性，该研究详细介绍了纤维基质如何能够灵敏并高速捕获抗原。就灵敏度而言，当前系统可与常规使用的平面PS基板相媲美。此外，当使用ESPS-真空系统时，在60秒内在60分钟内捕获到的平面PS底物上的抗原量就超过了5秒。然后注意到了三维ESPS纤维毡，作为Damkohler数与平面PS和ESPS-真空系统之间的比较。ESPS-真空系统的大流量允许达姆克勒数为0.37，表明流速和反应速率之间的平衡，而PS平板平台为5.80×10参照图4，其示出了扩散速率受限系统。最后，测试了整个ESPS-真空系统的免疫测定能力。在PS平面扩散和ESPS真空系统上均进行了基于三明治荧光的免疫测定。所述ESPS真空系统指示从5更宽的探测范围能力1000纳克毫升-1在5与PS平面扩散系统相比至100ng毫升-1。
更新日期：2017-12-22
中文翻译：
在大流量条件下快速有效地捕获抗原的静电纺基于聚合物超细纤维的平台的制造和评估†
这项研究调查了设计和概念设计的证明，这些演示证明了利用电纺聚苯乙烯（PS）超细纤维毡基质与真空泵加压配对以诱导大流量快速而高度敏感的抗原捕获。与用于免疫测定的常规平坦PS表面相比，该系统通过使用真空泵优化了电纺聚苯乙烯（ESPS）纤维垫基质的增加的表面积以及抗原通过检测平台的加速传播，从而实现了高效，快速抗原捕获。通过一项参数研究证明了这项工作的新颖性，该研究详细介绍了纤维基质如何能够灵敏并高速捕获抗原。就灵敏度而言，当前系统可与常规使用的平面PS基板相媲美。此外，当使用ESPS-真空系统时，在60秒内在60分钟内捕获到的平面PS底物上的抗原量就超过了5秒。然后注意到了三维ESPS纤维毡，作为Damkohler数与平面PS和ESPS-真空系统之间的比较。ESPS-真空系统的大流量允许达姆克勒数为0.37，表明流速和反应速率之间的平衡，而PS平板平台为5.80×10参照图4，其示出了扩散速率受限系统。最后，测试了整个ESPS-真空系统的免疫测定能力。在PS平面扩散和ESPS真空系统上均进行了基于三明治荧光的免疫测定。所述ESPS真空系统指示从5更宽的探测范围能力1000纳克毫升-1在5与PS平面扩散系统相比至100ng毫升-1。
京公网安备 11010802027423号