A label-free, versatile and low-background chemiluminescence aptasensing strategy based on gold nanocluster catalysis combined with the separation of magnetic beads†,Analyst

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A label-free, versatile and low-background chemiluminescence aptasensing strategy based on gold nanocluster catalysis combined with the separation of magnetic beads†
Analyst ( IF 3.6 ) Pub Date : 2017-12-22 00:00:00 , DOI: 10.1039/c7an01765k
Yueyue Yao _{1,

2,

3,

4} , Xiuzhong Wang _{1,

2,

3,

4} , Wenna Duan _{1,

2,

3,

4} , Feng Li _{1,

2,

3,

4}

Affiliation

A label-free, versatile and low-background chemiluminescence (CL) sensing strategy based on gold nanocluster catalysis combined with the separation of magnetic beads (MBs) was developed. Kanamycin was selected as the target analyte to exhibit the analytical performance of this platform. Two single-stranded DNA (named DNA1 and DNA2) are ingeniously designed. DNA1, containing an aptamer sequence of the targets, was firstly immobilized on the MBs which were modified with many amino groups by amidation reaction. DNA2 consists of 30 repeat adenosine bases (A₃₀) at the 5′ terminal which were used to prepare AuNCs by a UV-light-assisted method and a 12 nucleotide sequence at the 3′ terminal which can easily hybridize with DNA1 to form a partly complementary double-stranded structure. In the presence of targets, the aptamer modified on MBs would combine with targets and lead to release the prepared DNA-templated AuNCs. After the magnetic separation, enrichment AuNCs in the supernatant can catalyze the CL substrate to produce a strong CL signal. The well-designed CL sensing strategy exhibited a low detection limit of 0.035 nM for kanamycin, and it also showed good selectivity and stability. Most importantly, different targets can be analyzed only by changing the aptamer sequence that is immobilized on the MBs. Therefore, the strategy we proposed here has provided a versatile sensing platform for sensitively detecting various biomolecules at low levels.

中文翻译：

基于金纳米团簇催化并结合磁珠分离的无标签，通用和低背景化学发光适体策略†

开发了一种基于金纳米簇催化结合磁珠（MBs）分离的无标签，通用和低背景化学发光（CL）传感策略。选择卡那霉素作为目标分析物，以展示该平台的分析性能。巧妙地设计了两个单链DNA（分别称为DNA1和DNA2）。首先将含有靶标适体序列的DNA1固定在通过酰胺化反应修饰了许多氨基的MBs上。DNA2由30个重复的腺苷碱基组成（A ₃₀）在5'末端用于通过UV光辅助方法制备AuNCs，并且在3'末端具有12个核苷酸的序列，可以容易地与DNA1杂交形成部分互补的双链结构。在存在靶标的情况下，在MBs上修饰的适体将与靶标结合并导致释放制备的DNA模板化的AuNC。磁分离后，上清液中富集的AuNCs可以催化CL底物产生强CL信号。精心设计的CL传感策略对卡那霉素的检出限较低，为0.035 nM，并且还显示出良好的选择性和稳定性。最重要的是，只有通过改变固定在MBs上的适体序列，才能分析不同的靶标。所以，

更新日期：2017-12-22

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