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One-step coelectrodeposition-assisted layer-by-layer assembly of gold nanoparticles and reduced graphene oxide and its self-healing three-dimensional nanohybrid for an ultrasensitive DNA sensor†
Nanoscale ( IF 5.8 ) Pub Date : 2017-12-22 00:00:00 , DOI: 10.1039/c7nr06952a Jayakumar Kumarasamy 1, 2, 3, 4, 5 , María Belén Camarada 6, 7, 8, 9, 10 , Dharuman Venkatraman 1, 2, 3, 4 , Huangxian Ju 5, 11, 12, 13, 14 , Ramendra Sundar Dey 4, 15, 16 , Yangping Wen 17, 18, 19, 20
Nanoscale ( IF 5.8 ) Pub Date : 2017-12-22 00:00:00 , DOI: 10.1039/c7nr06952a Jayakumar Kumarasamy 1, 2, 3, 4, 5 , María Belén Camarada 6, 7, 8, 9, 10 , Dharuman Venkatraman 1, 2, 3, 4 , Huangxian Ju 5, 11, 12, 13, 14 , Ramendra Sundar Dey 4, 15, 16 , Yangping Wen 17, 18, 19, 20
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A layer-by-layer (LBL) assembly was employed for preparing multilayer thin films with a controlled architecture and composition. In this study, we report the one-step coelectrodeposition-assisted LBL assembly of both gold nanoparticles (AuNPs) and reduced graphene oxide (rGO) on the surface of a glassy carbon electrode (GCE) for the ultrasensitive electrochemical impedance sensing of DNA hybridization. A self-healable nanohybrid thin film with a three-dimensional (3D) alternate-layered nanoarchitecture was obtained by the one-step simultaneous electro-reduction of both graphene oxide and gold chloride in a high acidic medium of H2SO4 using cyclic voltammetry and was confirmed by different characterization techniques. The DNA bioelectrode was prepared by immobilizing the capture DNA onto the surface of the as-obtained self-healable AuNP/rGO/AuNP/GCE with a 3D LBL nanoarchitecture via gold–thiol interactions, which then served as an impedance sensing platform for the label-free ultrasensitive electrochemical detection of DNA hybridization over a wide range from 1.0 × 10−9 to 1.0 × 10−13 g ml−1, a low limit of detection of 3.9 × 10−14 g ml−1 (S/N = 3), ultrahigh sensitivity, and excellent selectivity. This study presents a promising electrochemical sensing platform for the label-free ultrasensitive detection of DNA hybridization with potential application in cancer diagnostics and the preparation of a self-healable nanohybrid thin film with a 3D alternate-layered nanoarchitecture via a one-step coelectrodeposition-assisted LBL assembly.
中文翻译：
金纳米粒子和还原的氧化石墨烯及其自愈三维纳米杂物的一步共电沉积辅助逐层组装，用于超灵敏DNA传感器†
采用逐层（LBL）组件来制备具有受控结构和组成的多层薄膜。在这项研究中，我们报告了玻璃碳电极（GCE）表面上的金纳米颗粒（AuNPs）和还原型氧化石墨烯（rGO）的一步电沉积辅助LBL组装，用于DNA杂交的超灵敏电化学阻抗传感。通过在高酸性H 2 SO 4介质中一步同时电还原氧化石墨烯和氯化金，获得了具有三维（3D）交替层纳米结构的自修复纳米杂化薄膜。使用循环伏安法并通过不同的表征技术得到证实。通过将捕获的DNA通过金-硫醇相互作用将捕获的DNA固定在具有3D LBL纳米结构的自修复AuNP / rGO / AuNP / GCE的表面上来制备DNA生物电极，然后将其用作标签的阻抗感应平台在1.0×10 -9至1.0×10 -13 g ml -1的宽范围内进行DNA杂交的超灵敏电化学检测，检测下限为3.9×10 -14 g ml -1（S / N = 3），超高灵敏度和出色的选择性。这项研究提供了一种有前途的电化学传感平台，可用于无标记的DNA杂交超灵敏检测，并有可能在癌症诊断中应用，并通过一步电沉积辅助制备具有3D交替层纳米结构的自修复纳米杂化薄膜。LBL组装。
更新日期：2017-12-22
中文翻译：
金纳米粒子和还原的氧化石墨烯及其自愈三维纳米杂物的一步共电沉积辅助逐层组装，用于超灵敏DNA传感器†
采用逐层（LBL）组件来制备具有受控结构和组成的多层薄膜。在这项研究中，我们报告了玻璃碳电极（GCE）表面上的金纳米颗粒（AuNPs）和还原型氧化石墨烯（rGO）的一步电沉积辅助LBL组装，用于DNA杂交的超灵敏电化学阻抗传感。通过在高酸性H 2 SO 4介质中一步同时电还原氧化石墨烯和氯化金，获得了具有三维（3D）交替层纳米结构的自修复纳米杂化薄膜。使用循环伏安法并通过不同的表征技术得到证实。通过将捕获的DNA通过金-硫醇相互作用将捕获的DNA固定在具有3D LBL纳米结构的自修复AuNP / rGO / AuNP / GCE的表面上来制备DNA生物电极，然后将其用作标签的阻抗感应平台在1.0×10 -9至1.0×10 -13 g ml -1的宽范围内进行DNA杂交的超灵敏电化学检测，检测下限为3.9×10 -14 g ml -1（S / N = 3），超高灵敏度和出色的选择性。这项研究提供了一种有前途的电化学传感平台，可用于无标记的DNA杂交超灵敏检测，并有可能在癌症诊断中应用，并通过一步电沉积辅助制备具有3D交替层纳米结构的自修复纳米杂化薄膜。LBL组装。
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