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Electrochemical synthesis of silver nanoclusters on electrochemiluminescent resonance energy transfer amplification platform for Apo-A1 detection
Talanta ( IF 5.6 ) Pub Date : 2017-12-22 , DOI: 10.1016/j.talanta.2017.12.063
Ying Zhou , Yanqing Yu , Yaqin Chai , Ruo Yuan

Herein, a novel electrochemiluminescence resonance energy transfer (ERET) strategy between in situ electrogenerated silver nanoclusters (AgNCs) as the donor and Ru(bpy)2(5-NH2-1,10-phen)Cl2 (Ru(II)) as the acceptor was reported, which was applied for the construction of a ultrasensitive immunosensor for the determination of apolipoprotein-A1 (Apo-A1). Notably, the Ag NCs were in suit electro-reducted on glassy carbon electrode (GCE) based on a simple, fast and controllable electrochemical technique, which acted as not only biocompatible ECL emitters, but also protein immobilization platform via Ag–N or Ag–S bond. Subsequently, the acceptor probe was constructed by modifying Ru(II) and secondary antibody on the nanocarriers of carboxyl-functionalized MWCNTs. Based on the sandwiched immunoassay method, the ECL signals declining at 449 nm (Ag NCs) and the increasing at 650 nm (Ru(II)) both reflect the amount of Apo-A1. By measuring the ratio of ECL650 nm/ECL449 nm, we could accurately quantify the concentration of Apo-A1 in range from 1.0 pg/mL to 1.0 ng/mL and limit of detection down to 0.33 pg/mL, which opened up a new research direction for ultrasensitive ECL bioanalysis based metal NCs.



中文翻译:

用于Apo-A1检测的电化学发光共振能量转移放大平台上的电化学合成银纳米簇

在这里,一种新的电化学发光共振能量转移(ERET)策略之间原位电生银纳米团簇(AgNCs)作为供体和Ru(bpy)2(5-NH 2 -1,10-phen)Cl 2报道了(Ru(II))作为受体,其被用于构建用于测定载脂蛋白A1(Apo-A1)的超灵敏免疫传感器。值得注意的是,基于一种简单,快速且可控制的电化学技术,Ag NCs适合在玻璃碳电极(GCE)上进行电解还原,该技术不仅可以作为生物相容性ECL发射体,还可以通过Ag–N或Ag–作为蛋白质固定平台S键。随后,通过修饰Ru(II)和二级抗体在羧基官能化MWCNTs纳米载体上构建受体探针。基于夹心免疫测定方法,ECL信号在449 nm处下降(Ag NCs)和在650 nm处增加(Ru(II))均反映Apo-A1的量。通过测量ECL 650 nm / ECL 449 nm的比率,我们可以准确定量Apo-A1的浓度,范围从1.0 pg / mL到1.0 ng / mL,检测极限低至0.33 pg / mL,这为基于超灵敏ECL生物分析的金属NC开辟了新的研究方向。

更新日期:2017-12-22
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