Herein, a novel methodology for ultrasensitive and facile breast cancer biomarker carbohydrate antigen 15-3 (CA15-3) evaluation was proposed by fabricating a sandwiched ECL immunosensor. In the protocol, MOCs-Fc and [email protected] nanohybrids were successfully synthesized and further employed to achieve dual-amplification strategy for luminol-H₂O₂ system. Notably, inherent porous microstructure and large specific surface area from MOCs enabled a high loading of Fc, which succeeded in catalyzing luminol-H₂O₂ ECL emission and therefore enhancing ECL response. In addition, higher sensitivity could be realized due to the excellent electronic conductivity of MOCs. Furthermore, the as-obtained [email protected] luminol was not only employed as capture probe to recognize CA15-3 after hybridization with Ab₂, but also played a crucial role in acting as ECL signal probe due to the presence of massive luminol. It is of vital importance that [email protected] core/shell nanospheres showed admirable catalytic effect towards H₂O₂, which resulted in more excited state luminol and stronger ECL intensity. Therefore, the synergistic amplification strategy of MOCs-Fc and [email protected] nanohybrids offered an extremely enhanced ECL signal. The well-established applicable ECL immune- sensing platform displayed favorable analytical performance for CA15-3. In summary, the proposed ECL immunosensor opened a new era for sensitive CA15-3 evaluation and offered a promising platform for clinical breast cancer diagnostics.

在此，通过制造夹心式ECL免疫传感器，提出了一种用于评估超敏和便捷的乳腺癌生物标志物碳水化合物抗原15-3（CA15-3）的新方法。在该方案中，成功合成了MOCs-Fc和[受电子邮件保护的]纳米杂交体，并进一步用于实现luminol-H ₂ O ₂系统的双重扩增策略。值得注意的是，MOC固有的多孔微结构和较大的比表面积使Fc高负载，从而成功催化了鲁米诺-H ₂ O ₂ECL排放，因此增强了ECL响应。此外，由于MOC具有出色的电子导电性，因此可以实现更高的灵敏度。此外，如此获得的[电子邮件保护的]鲁米诺不仅用作与Ab ₂杂交后识别CA15-3的捕获探针，而且由于大量鲁米诺的存在，在充当ECL信号探针中也起着至关重要的作用。[受电子邮件保护的]核/壳纳米球对H ₂ O ₂表现出令人钦佩的催化作用至关重要，从而导致更亮的鲁米诺状态和更强的ECL强度。因此，MOCs-Fc和[受电子邮件保护的]纳米杂交体的协同扩增策略提供了极大增强的ECL信号。完善的适用ECL免疫传感平台显示出对CA15-3的良好分析性能。总之，提出的ECL免疫传感器为敏感的CA15-3评估开辟了新纪元，并为临床乳腺癌诊断提供了有希望的平台。