Enzyme-linked immunosorbent assay (ELISA) is one of the most important technologies for biochemical analysis critical for diagnosis and monitoring of many diseases. Traditional systems for ELISA incubation and reading are expensive and bulky, thus cannot be used at point-of-care or in the field. Here, we propose and demonstrate a new miniature mobile phone based system for ELISA (MELISA). This system can be used to complete all steps of the assay, including incubation and reading. It weighs just 1 pound, can be fabricated at low cost, portable, and can transfer test results via mobile phone. We successfully demonstrated how MELISA can be calibrated for accurate measurements of progesterone and demonstrated successful measurements with the calibrated system.

酶联免疫吸附测定（ELISA）是生化分析最重要的技术之一，对许多疾病的诊断和监测至关重要。用于ELISA孵育和读取的传统系统昂贵且笨重，因此无法在现场或现场使用。在这里，我们提出并演示了一种新的基于微型手机的ELISA系统（MELISA）。该系统可用于完成测定的所有步骤，包括孵育和读取。它的重量仅为1磅，可以低成本，轻便地制造，并且可以通过手机传输测试结果。我们成功地演示了如何对MELISA进行校准以准确测量孕酮，并演示了使用校准后的系统成功进行的测量。