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Heparin‐gold Nanoparticles and Liquid Crystal Applied in Label‐free Electrochemical Immunosensor for Prostate‐specific Antigen
Electroanalysis ( IF 2.7 ) Pub Date : 2017-12-21 , DOI: 10.1002/elan.201700651 Lucas Talamini 1 , Nicole Zanato 1 , Eduardo Zapp 2 , Daniela Brondani 2 , Eduard Westphal 3 , Hugo Gallardo 1 , Iolanda Cruz Vieira 1
Electroanalysis ( IF 2.7 ) Pub Date : 2017-12-21 , DOI: 10.1002/elan.201700651 Lucas Talamini 1 , Nicole Zanato 1 , Eduardo Zapp 2 , Daniela Brondani 2 , Eduard Westphal 3 , Hugo Gallardo 1 , Iolanda Cruz Vieira 1
Affiliation
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A label‐free electrochemical immunosensor based on the liquid crystal (E)‐1‐decyl‐4‐[(4‐decyloxyphenyl)diazenyl]pyridinium bromide (Br−Py), together with heparin‐stabilized gold nanoparticles (AuNP‐Hep) and Nafion is proposed for the determination of prostate‐specific antigen (PSA). The Br−Py liquid crystal presented redox properties and good film‐forming abilities on the electrode surface, and thus it is a suitable alternative as a redox probe for a label‐free electrochemical immunosensor, which could simplify the analysis methodology. The stepwise construction of the immunosensor and the incubation process (immunocomplex formation) were characterized by voltammetry and electrochemical impedance spectroscopy. The proposed immunosensor could directly detect PSA concentrations in the incubation samples, based on the suppression of the Br−Py redox peak (‘base peak’) current. After optimization, the immunosensor exhibited a linear response to PSA concentrations in the range of 0.1 to 50 ng mL−1, with a calculated detection limit of 0.08 ng mL−1. The reproducibility (coefficient of variance less than 3.0 %), selectivity and accuracy of the methodology were adequate. The immunosensor was satisfactorily applied in the quantification of PSA in human blood plasma samples.
中文翻译：
肝素金纳米粒子和液晶技术在无标签电化学免疫传感器中用于前列腺特异性抗原的应用
基于液晶（E）-1-癸基-4-[[（4-癸氧基苯基）二氮烯基]溴化吡啶鎓（Br-Py）以及肝素稳定的金纳米颗粒（AuNP-Hep）的无标签电化学免疫传感器Nafion被提议用于测定前列腺特异性抗原（PSA）。Br-Py液晶在电极表面上具有氧化还原特性和良好的成膜能力，因此它是无标记电化学免疫传感器的氧化还原探针的合适替代品，可以简化分析方法。通过伏安法和电化学阻抗谱对免疫传感器的逐步构建和孵育过程（免疫复合物的形成）进行了表征。所提出的免疫传感器可以直接检测孵育样品中的PSA浓度，基于对Br-Py氧化还原峰（“基峰”）电流的抑制。经过优化后，免疫传感器对PSA浓度在0.1至50 ng mL范围内表现出线性响应-1，计算出的检出限为0.08 ng mL -1。该方法的重现性（方差系数小于3.0％），选择性和准确性均足够。该免疫传感器可令人满意地用于定量人体血浆样品中的PSA。
更新日期：2017-12-21
中文翻译：
肝素金纳米粒子和液晶技术在无标签电化学免疫传感器中用于前列腺特异性抗原的应用
基于液晶（E）-1-癸基-4-[[（4-癸氧基苯基）二氮烯基]溴化吡啶鎓（Br-Py）以及肝素稳定的金纳米颗粒（AuNP-Hep）的无标签电化学免疫传感器Nafion被提议用于测定前列腺特异性抗原（PSA）。Br-Py液晶在电极表面上具有氧化还原特性和良好的成膜能力，因此它是无标记电化学免疫传感器的氧化还原探针的合适替代品，可以简化分析方法。通过伏安法和电化学阻抗谱对免疫传感器的逐步构建和孵育过程（免疫复合物的形成）进行了表征。所提出的免疫传感器可以直接检测孵育样品中的PSA浓度，基于对Br-Py氧化还原峰（“基峰”）电流的抑制。经过优化后，免疫传感器对PSA浓度在0.1至50 ng mL范围内表现出线性响应-1，计算出的检出限为0.08 ng mL -1。该方法的重现性（方差系数小于3.0％），选择性和准确性均足够。该免疫传感器可令人满意地用于定量人体血浆样品中的PSA。
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