Facile Synthesis of Lipid–Perfluorocarbon Nanoemulsion Coated with Silica Shell as an Ultrasound Imaging Agent,Advanced Healthcare Materials

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Facile Synthesis of Lipid–Perfluorocarbon Nanoemulsion Coated with Silica Shell as an Ultrasound Imaging Agent
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-12-21 , DOI: 10.1002/adhm.201700816
Qiang Yang ₁ , Huali Chen ₁ , Yan Bai ₁ , Yang Cao ₂ , Wenjing Hu ₃ , Liangke Zhang ₁

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A novel organic/inorganic hybrid nanovesicle as an ultrasound imaging agent is synthesized via facile emulsion and silica deposition methods. This nanovesicle, hyaluronate (HA)–docetaxel (DTX)/perfluoro‐n‐pentane (PFP)@SNC, consists of an encapsulated liquid PFP core, loaded DTX, and an HA‐decorated silica shell. The HA–DTX/PFP@SNC has a narrow size distribution of 274.5 ± 3.25 nm, a negative zeta potential of −11.6 ± 0.47 mV, and an entrapment efficiency of 86.70% ± 1.42%. HA–DTX/PFP@SNC possesses an ultrasound (US)‐triggered drug release and a temperature‐dependent size change behavior. Compared with DTX/PFP@soybean phosphatidylcholine (SPC), which has no silica shell, the HA–DTX/PFP@SNC is more stable under various conditions. The MTT assay indicates that the blank HA–PFP@SNC vehicle has no cytotoxicity to A549 cells. Furthermore, due to the HA‐mediated tumor‐targeting ability, the HA–DTX/PFP@SNC shows obvious cytotoxicity to A549 cells. In vitro and in vivo US imaging results indicate that HA–DTX/PFP@SNC has a stronger and more durable echo signal than DTX/PFP@SPC. Moreover, the in vivo echo signal of HA–DTX/PFP@SNC is stronger than that of DTX/PFP@SNC due to the HA‐mediated tumor targeting. Therefore, this novel organic/inorganic hybrid vesicle is a US contrast agent candidate.

中文翻译：

硅胶壳包覆超声成像剂脂质-全氟化碳纳米乳液的合成

通过简便的乳液和二氧化硅沉积方法合成了一种新型的有机/无机杂化纳米囊泡作为超声显像剂。此纳米囊泡，透明质酸（HA） -多烯紫杉醇（DTX）/全氟Ñ戊烷（PFP）@SNC，由封装的液态PFP芯，负载的DTX和HA装饰的二氧化硅外壳组成。HA–DTX / PFP @ SNC具有274.5±3.25 nm的窄尺寸分布，-11.6±0.47 mV的负Zeta电位和86.70％±1.42％的包封效率。HA–DTX / PFP @ SNC具有超声（美国）触发的药物释放和随温度变化的尺寸变化行为。与没有二氧化硅壳的DTX / PFP @大豆磷脂酰胆碱（SPC）相比，HA–DTX / PFP @ SNC在各种条件下都更稳定。MTT分析表明空白的HA–PFP @ SNC载体对A549细胞没有细胞毒性。此外，由于HA介导的肿瘤靶向能力，HA–DTX / PFP @ SNC对A549细胞显示出明显的细胞毒性。体外和体内美国成像结果表明，HA-DTX / PFP @ SNC比DTX / PFP @ SPC具有更强，更持久的回声信号。此外，由于HA介导的肿瘤靶向作用，HA–DTX / PFP @ SNC的体内回声信号比DTX / PFP @ SNC的体内回波信号强。因此，这种新颖的有机/无机杂化囊泡是美国造影剂的候选者。

更新日期：2017-12-21

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