Can strand displacement take place in DNA triplexes?†,Organic & Biomolecular Chemistry

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Can strand displacement take place in DNA triplexes?†
Organic & Biomolecular Chemistry ( IF 2.9 ) Pub Date : 2017-12-21 00:00:00 , DOI: 10.1039/c7ob02871g
Qian Li _{1,

2,

3,

4} , Cheng Tian _{1,

2,

3,

4} , Xiang Li _{1,

2,

3,

4} , Chengde Mao _{1,

2,

3,

4}

Affiliation

This work studies strand displacement in the context of DNA triplexes. We observed that toehold-mediated strand displacement could proceed in DNA triplexes, but at a much slower rate than in DNA duplexes. In this study, strand displacement was studied with native polyacrylamide gel electrophoresis (PAGE) for two molecular systems. While both systems were involved in branch migration in a DNA triplex, the toehold forms either a duplex or a triplex. This study provides a new strategy for sequence-specific strand displacement and it is useful for the construction of dynamic DNA nanostructures and control of smart DNA nanodevices.

中文翻译：

DNA三链体中会发生链置换吗？†

这项工作研究DNA三链体的背景下链置换。我们观察到脚趾介导的链置换可以在DNA三链体中进行，但速率要比DNA双链体慢得多。在这项研究中，使用天然聚丙烯酰胺凝胶电泳（PAGE）研究了两个分子系统的链置换。虽然两个系统都参与DNA三链体的分支迁移，但脚尖形成双链体或三链体。这项研究为序列特异性链置换提供了一种新的策略，对于动态DNA纳米结构的构建和智能DNA纳米设备的控制非常有用。

更新日期：2017-12-21

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