Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles†,Environmental Science: Nano

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Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles†
Environmental Science: Nano ( IF 5.8 ) Pub Date : 2017-12-20 00:00:00 , DOI: 10.1039/c7en00832e
Joseph T. Buchman _{1,

2,

3,

4} , Ali Rahnamoun _{1,

4,

5,

6} , Kaitlin M. Landy _{1,

2,

3,

4} , Xi Zhang _{1,

4,

7,

8} , Ariane M. Vartanian _{1,

4,

7,

8} , Lisa M. Jacob _{1,

4,

7,

8} , Catherine J. Murphy _{1,

4,

7,

8} , Rigoberto Hernandez _{1,

4,

5,

6} , Christy L. Haynes _{1,

2,

3,

4}

Affiliation

We aim to establish the effect of environmental diversity in evaluating nanotoxicity to bacteria. We assessed the toxicity of 4 nm polyallylamine hydrochloride-wrapped gold nanoparticles (PAH AuNPs) to a panel of bacteria from diverse environmental niches. The bacteria experienced a range of toxicities as evidenced by the different minimum bactericidal concentrations determined; the sensitivities of the bacteria was A. vinelandii = P. aeruginosa > S. oneidensis MR-4 > A. baylyi > S. oneidensis MR-1. Interactions between gold nanoparticles and molecular components of the cell wall were investigated by TEM, flow cytometry, and computational modeling. Binding results showed a general trend that bacteria with smooth lipopolysaccharides (LPS) bind more PAH AuNPs than bacteria with rough LPS. Computational models reveal that PAH migrates to phosphate groups in the core of the LPS structure. Overall, our results demonstrate that simple interactions between nanoparticles and the bacterial cell wall cannot fully account for observed trends in toxicity, which points to the importance of establishing more comprehensive approaches for modeling environmental nanotoxicity.

中文翻译：

使用与环境有关的革兰氏阴性菌组评估聚烯丙胺盐酸盐包裹的金纳米颗粒的毒性†

我们旨在建立环境多样性在评估细菌纳米毒性方面的作用。我们评估了4 nm聚烯丙胺盐酸盐包裹的金纳米颗粒（PAH AuNPs）对来自各种环境生态位的细菌的毒性。通过确定的不同最低杀菌浓度可以证明细菌具有多种毒性。细菌的敏感性为：A。vinelandii =铜绿假单胞菌>拟南芥MR-4> A. baylyi >拟南芥MR-1。通过TEM，流式细胞仪和计算模型研究了金纳米颗粒与细胞壁分子成分之间的相互作用。结合结果表明，一般趋势是，具有光滑脂多糖（LPS）的细菌比具有粗糙LPS的细菌结合更多的PAH AuNP。计算模型表明，PAH迁移到LPS结构核心的磷酸基团。总体而言，我们的结果表明，纳米颗粒与细菌细胞壁之间的简单相互作用无法完全解释所观察到的毒性趋势，这表明建立更全面的方法来模拟环境纳米毒性的重要性。

更新日期：2017-12-20

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