ARDRA analysis was carried out on 90 New Zealand psychrotolerant Clostridium isolates derived from three meat production animal types and their environments. The isolates included species associated with spoilage: C. gasigenes, C. algidicarnis, C. tagluense, C. frigidicarnis and C. estertheticum. The isolates fell into 14 distinct ARDRA Groups, with 13 previously characterised meat spoilage-associated isolates shared between 6 of the 14 groups. The accuracy of ARDRA profiling analysis was supported by sequencing the 16s rRNA gene from isolates, including the representative spoilage associated Clostridium species and was consistent with previous phylogenetic relationships and classical cultural characterisation. The ARDRA methodology described in this study successfully discriminated between the different spoilage-associated species of clostridia as well as other pyschrotolerant Clostridium species associated with meat production. This discriminatory molecular screen will aid future source attribution studies as well as enable meat processors to identify and validate control measures for clostridia contamination, thus gaining greater efficacy in controlling meat spoilage caused by psychrotolerant clostridia.

ARDRA分析是对90种来自三种肉类生产动物及其环境的新西兰抗精神性梭状芽胞杆菌分离株进行的。分离物包括与腐败相关的物种：C。gasigenes，C。algidicarnis，C。tagluense，C。frigidicarnis和C. estertheticum。这些分离物分为14个不同的ARDRA组，在14个组中的6个之间共有13个先前表征的与肉腐败相关的分离物。通过对分离株（包括代表性的腐败相关梭状芽胞杆菌）中的16s rRNA基因进行测序，可以支持ARDRA分析分析的准确性。物种，并与以前的系统发育关系和古典文化特征保持一致。这项研究中描述的ARDRA方法成功地区分了与腐败相关的梭状芽胞杆菌不同种类以及与肉类生产相关的其他耐丙酮酸梭状芽孢杆菌种类。这种具有歧视性的分子筛查将有助于未来的来源归因研究，并使肉类加工者能够识别和验证针对梭菌污染的控制措施，从而在控制由精神耐受性梭菌引起的肉类腐败中获得更大的功效。