In this study, we designed a gadolinium-based immobilized metal ion affinity chromatography material for the selective enrichment of phosphopeptides. Gadolinium ion was immobilized on the surface of glutathione-coated magnetic nanoparticles through a facile and effective synthetic route. The adsorbent integrated the advantages of superparamagnetism of Fe₃O₄ core, good biological compatibility of glutathione, and strong interaction between gadolinium ion and phosphopeptides. It was employed to enrich phosphopeptides from standard protein digests coupled with MALDI-TOF MS. Results demonstrated that the adsorbent possessed high selectivity for phosphopeptides, good reusability and reproducibility. Moreover, the material provided selective enrichment of phosphopeptides from real samples including non-fat milk digests and human serum. The developed method exhibited high sensitivity (detection limit of 10 fmol), showing great potential in the detection of low-abundance phosphopeptides in biological samples.

在这项研究中，我们设计了一种基于lin的固定化金属离子亲和色谱材料，以选择性富集磷酸肽。通过简便有效的合成途径，将route离子固定在谷胱甘肽涂层的磁性纳米颗粒的表面上。吸附剂整合了Fe ₃ O ₄超顺磁性的优点核心，谷胱甘肽的良好生物相容性以及g离子与磷酸肽之间的强相互作用。它用于与MALDI-TOF MS结合从标准蛋白质消化物中富集磷酸肽。结果表明，该吸附剂具有对磷肽的高选择性，良好的可重复使用性和可再现性。此外，该材料可从包括脱脂牛奶消化物和人血清在内的真实样品中选择性富集磷酸肽。所开发的方法具有很高的灵敏度（检测极限为10 fmol），在生物样品中低丰度磷酸肽的检测中显示出巨大的潜力。