Dairy product recalls and dairy-related illnesses are often the result of contamination with Listeria monocytogenes, which can occur throughout the dairy production and supply chains. The use of antimicrobial compounds is one practical approach for controlling pathogen survival and growth in foods. The goal of this study was to use fluid milk as a model system to identify listeristatic or listericidal treatments that show promise for application in fluid milk and for further evaluation in other dairy products (e.g., cheese). Caprylic acid (CA), ε-polylysine (EPL), hydrogen peroxide, lauric arginate (LAE), and sodium caprylate (SC) were added individually or in combination to whole milk inoculated with L. monocytogenes at ~4 log₁₀ cfu/mL. Samples were stored at 7°C for 21 d, and L. monocytogenes counts were determined weekly. Inhibitory concentrations of LAE (800 mg/L) and EPL (100–400 mg/L), as well as SC and CA (3,200 mg/L each), were identified. The addition of EPL at 800 mg/L reduced L. monocytogenes counts by >3 log₁₀ cfu/mL from initial inoculation levels after 21 d. Addition of hydrogen peroxide to milk reduced counts by >3 log₁₀ cfu/mL from initial inoculation within 24 h (400 and 800 mg/L) or by d 7 (200 mg/L). Although the combinatory treatments of EPL + CA, EPL + LAE, and LAE + SC were characterized as indifferent, EPL + SC worked synergistically to reduce L. monocytogenes populations in milk over 21 d. Overall, these data identify potential antimicrobial treatments to control L. monocytogenes in milk and serve as a foundation for the continued development of antimicrobial controls for L. monocytogenes in dairy products.

乳制品召回和与乳制品有关的疾病通常是单核细胞增生性李斯特菌污染的结果，李斯特菌污染可能发生在整个乳制品生产和供应链中。抗菌化合物的使用是控制食品中病原体存活和生长的一种实用方法。这项研究的目的是使用液态奶作为模型系统，以鉴定显示出有希望在液态奶中应用和在其他乳制品（例如奶酪）中进行进一步评估的潜力的李斯特抑菌或杀菌方法。将辛酸（CA），ε-聚赖氨酸（EPL），过氧化氢，精氨酸月桂酸酯（LAE）和辛酸钠（SC）单独或组合添加到接种单核细胞增生李斯特氏菌的全脂牛奶中，约4 log ₁₀cfu / mL。将样品在7°C下保存21天，每周测定一次单核细胞增生李斯特菌计数。确定了LAE（800 mg / L）和EPL（100-400 mg / L）以及SC和CA（各自为3200 mg / L）的抑制浓度。加入800 mg / L的EPL可使单核细胞增生李斯特菌计数从21 d后的初始接种水平降低> 3 log ₁₀ cfu / mL。牛奶中添加过氧化氢后，从最初接种24小时（400和800 mg / L）开始到接种后24小时（400和800 mg / L）减少计数> 3 log ₁₀ cfu / mL，或者减少d 7（200 mg / L）。尽管EPL + CA，EPL + LAE和LAE + SC的联合治疗被认为是无关紧要的，但EPL + SC协同作用以减少单核细胞增生李斯特氏菌超过21天的牛奶数量。总体而言，这些数据确定了控制牛奶中单核细胞增生李斯特氏菌的潜在抗微生物治疗方法，并为继续开发乳制品中单核细胞增生李斯特氏菌的抗菌控制奠定了基础。