Milk mineral content is a key trait for its role in dairy processes such as cheese-making, its use as source of minerals for newborns, and for all traits involving salt-protein interactions. This study investigated a new method for measuring mineral partition between soluble and micellar fractions in bovine milk after rennet coagulation. A new whey dilution step was added to correct the quantification bias due to whey trapped in curd and excluded volume. Moreover, the proposed method allowed the quantification of the diffusible volume after milk coagulation. Milk mineral content and concentration in whey, and diluted whey were quantified by acid digestion and inductively coupled plasma optical emission spectrometry. The repeatability of the method for micellar Ca, Mg, and K was between 2.07 and 8.96%, whereas reproducibility ranged from 4.01 to 9.44%. Recovery of total milk minerals over 3 spiking levels ranged from 92 to 97%. The proposed method provided an accurate estimation of micellar and soluble minerals in milk, and curd diffusible volume.

牛奶中的矿物质含量是其在乳制品加工（例如奶酪制作）中的作用，其作为新生儿矿物质来源的用途以及涉及盐-蛋白质相互作用的所有特征的关键特征。这项研究研究了一种新方法，用于测量凝乳酶凝结后牛乳中可溶性和胶束级分之间的矿物质分配。添加了新的乳清稀释步骤，以纠正由于乳清滞留在凝乳中并排除体积而导致的定量偏差。而且，所提出的方法允许定量牛奶凝结后的可扩散体积。乳清液中乳矿物质含量和浓度以及稀释的乳清液通过酸消解和电感耦合等离子体发射光谱法进行定量。该方法对胶束Ca，Mg和K的重现性在2.07至8.96％之间，而重现性在4.01至9之间。44％。超过3种加标水平的总牛奶矿物质回收率在92％至97％之间。所提出的方法可以准确估算牛奶中的胶束和可溶性矿物质，以及凝乳的可扩散体积。