An optoelectronic sensor is a rapid diagnostic tool that allows for an accurate, reliable, field-portable, low-cost device for practical applications. In this study, template-free In situ gold nanobundles (Au NBs) were fabricated on an electrode for optoelectronic sensing of fowl adenoviruses (FAdVs). Au NB film was fabricated on carbon electrodes working area using L(+) ascorbic acid, gold chroloauric acid and poly-l-lysine (PLL) through modified layer-by-layer (LbL) method. A scanning electron microscopic (SEM) image of the Au NBs revealed a NB-shaped Au structure with many kinks on its surface, which allow local electric field enhancement through light–matter interaction with graphene quantum dots (GQDs). Here, GQDs were synthesized through an autoclave-assisted method. Characterization experiments revealed blue-emissive, well-dispersed GQDs that were 2–3 nm in size with the fluorescence emission peak of GQDs located at 405 nm. Both Au NBs and GQDs were conjugated with target FAdVs specific antibodies that bring them close to each other with the addition of target FAdVs through antibody–antigen interaction. At close proximity, light–matter interaction between Au NBs and QDs produces a local electric signal enhancement under Ultraviolet–visible (UV–visible) light irradiation that allows the detection of very low concentrations of target virus even in complex biological media. A proposed optoelectronic sensor showed a linear relationship between the target FAdVs and the electric signal up to 10 Plaque forming unit (PFU)/mL with a limit of detection (LOD) of 8.75 PFU/mL. The proposed sensing strategy was 100 times more sensitive than conventional ELISA method.

光电传感器是一种快速诊断工具，可为实际应用提供准确，可靠，可现场移植的低成本设备。在这项研究中，无模板的原位金纳米束（Au NBs）被制作在用于禽腺病毒（FAdVs）的光电传感的电极上。使用L（+）抗坏血酸，金氯金酸和聚-l在碳电极工作区上制备金纳米管薄膜-赖氨酸（PLL）通过改进的逐层（LbL）方法。金纳米粒子的扫描电子显微镜（SEM）图像显示出其表面上有许多纽结的NB形金结构，通过与石墨烯量子点（GQD）的光-物质相互作用，可以增强局部电场。在这里，GQD是通过高压釜辅助方法合成的。表征实验显示，蓝色发光，分散良好的GQD大小为2–3 nm，GQD的荧光发射峰位于405 nm。Au NBs和GQDs均与目标FAdVs特异性抗体偶联，通过抗体-抗原相互作用添加目标FAdVs，使它们彼此靠近。在附近，金纳米颗粒和量子点之间的光-物质相互作用在紫外线-可见（UV-可见）光照射下产生局部电信号增强，从而即使在复杂的生物介质中也可以检测到极低浓度的目标病毒。拟议的光电传感器显示目标FAdVs和电信号之间的线性关系高达10噬斑形成单位（PFU）/ mL，检测极限（LOD）为8.75 PFU / mL。所提出的传感策略的灵敏度是传统ELISA方法的100倍。