Ochratoxin A (OTA) is a mycotoxin produced by the metabolism of fungus belonging to the genus Aspergillus and Penicillium. In this paper we report, the capacity of different cultures of lactic acid bacteria (LAB) to degrade OTA present in MRS broth at both pH 3.5 and 6.5. A study of OTA reduction during gastrointestinal digestion carried out with the LAB was also performed. Taking into account the two reduction mechanisms of OTA studied in this work as the enzymatic one and the adsorption on the cell wall, as well as at pH 3.5 and 6.5 the reduction values of OTA were in a range of 30–99%, being the strains with greater reduction (97% and 95%) Lb. rhamnosus CECT 278T and Lb. plantarum CECT 749 respectively. In the experiments carried out digesting the OTA in MRS medium with LAB, the highest bioaccessibility reduction was observed by the strain of Lb. johnsonii CECT 289, showing a mean reduction around all the gastrointestinal digestion process of 97.4%. The mass spectrometry associated to the linear ion trap method identified ochratoxin alpha (OTα) m/z = 256.1 and phenylalanine (Phe) m/z = 166.1 as the major metabolites of OTA degradation in LAB cultures.

ch曲霉毒素A（OTA）是一种霉菌毒素，由曲霉菌和青霉菌属的真菌代谢产生。在本文中，我们报告了乳酸菌（LAB）的不同培养物降解pH值为3.5和6.5的MRS肉汤中OTA的能力。还对使用LAB进行的胃肠道消化过程中OTA的降低进行了研究。考虑到在这项工作中研究的两种OTA还原机制，即酶促还原和在细胞壁上的吸附，以及在pH 3.5和6.5时，OTA的还原值在30-99％的范围内，即降低Lb的菌株（97％和95％）。鼠李糖CECT 278T和Lb. 植物CECT 749分别。在用LAB消化MRS培养基中的OTA的实验中，Lb菌株观察到最大的生物可及性降低。johnsonii CECT 289，显示所有胃肠消化过程的平均减少量为97.4％。与线性离子阱方法相关的质谱分析确定曲毒素α（OTα）m / z  = 256.1和苯丙氨酸（Phe）m / z  = 166.1是LAB培养物中OTA降解的主要代谢产物。