Single-Droplet Multiplex Bioassay on a Robust and Stretchable Extreme Wetting Substrate through Vacuum-Based Droplet Manipulation,ACS Nano

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Single-Droplet Multiplex Bioassay on a Robust and Stretchable Extreme Wetting Substrate through Vacuum-Based Droplet Manipulation
ACS Nano ( IF 15.8 ) Pub Date : 2018-01-16 00:00:00 , DOI: 10.1021/acsnano.7b05826
Heetak Han , Jung Seung Lee , Hyunchul Kim , Sera Shin , Jaehong Lee , Jongchan Kim ₁ , Xu Hou ₂ , Seung-Woo Cho , Jungmok Seo _{3,

4} , Taeyoon Lee

Affiliation

Herein, a droplet manipulation system with a superamphiphobic (SPO)–superamphiphilic (SPI) patterned polydimethylsiloxane (PDMS) substrate is developed for a multiplex bioassay from single-droplet samples. The SPO substrate is fabricated by sequential spraying of adhesive and fluorinated silica nanoparticles onto a PDMS substrate. It is subsequently subjected to oxygen plasma with a patterned mask to form SPI patterns. The SPO layer exhibits extreme liquid repellency with a high contact angle (>150°) toward low surface tension and viscous biofluidic droplets (e.g., ethylene glycol, blood, dimethyl sulfoxide, and alginate hydrogel). In contrast, the SPI exhibits liquid adhesion with a near zero contact angle. Using the droplet manipulation system, various liquid droplets can be precisely manipulated and dispensed onto the predefined SPI patterns on the SPO PDMS substrate. This system enables a multiplex colorimetric bioassay, capable of detecting multiple analytes, including glucose, uric acid, and lactate, from a single sample droplet. In addition, the detection of glucose concentrations in a plasma droplet of diabetic and healthy mice are performed to demonstrate the feasibility of the proposed system for efficient clinical diagnostic applications.

中文翻译：

通过基于真空的液滴操作，在坚固且可拉伸的极端润湿基质上进行单滴多重生物测定

本文中，开发了一种具有超两亲（SPO）-超两亲（SPI）图案化聚二甲基硅氧烷（PDMS）底物的液滴操纵系统，用于从单个液滴样品进行多重生物测定。通过将粘合剂和氟化二氧化硅纳米粒子顺序喷涂到PDMS基板上，可以制造SPO基板。随后，使用图案化的掩模对其进行氧等离子体处理，以形成SPI图案。SPO层具有极高的拒液性，并且具有高的接触角（> 150°），朝向低的表面张力和粘性的生物流体小滴（例如，，乙二醇，血液，二甲基亚砜和藻酸盐水凝胶）。相反，SPI表现出接近零接触角的液体粘附力。使用液滴操纵系统，可以精确操纵各种液滴并将其分配到SPO PDMS基板上的预定义SPI图案上。该系统能够进行多重比色生物测定，能够从单个样品滴中检测多种分析物，包括葡萄糖，尿酸和乳酸。另外，进行糖尿病和健康小鼠血浆滴中葡萄糖浓度的检测以证明所提出的系统用于有效临床诊断应用的可行性。

更新日期：2018-01-16

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