Identification and Characterization of Diacylglycerol Acyltransferase from Oleaginous Fungus Mucor circinelloides,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Identification and Characterization of Diacylglycerol Acyltransferase from Oleaginous Fungus Mucor circinelloides
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-10 00:00:00 , DOI: 10.1021/acs.jafc.7b04295
Luning Zhang _{1,

2} , Huaiyuan Zhang ₁ , Yuanda Song ₁

Affiliation

Acyl-CoA:diacylglycerol acyltransferase (DGAT) is a pivotal regulator of triacylglycerol (TAG) synthesis. The oleaginous fungus Mucor circinelloides has four putative DGATs: McDGAT1A, McDGAT1B, McDGAT2A, and McDGAT2B, classified into the DGAT1 and DGAT2 subfamilies, respectively. To identify and characterize DGATs in M. circinelloides, these four genes were expressed in Saccharomyces cerevisiae H1246 (TAG-deficient quadruple mutant), individually. TAG biosynthesis was restored only by the expression of McDGAT2B, and TAG content was significantly higher in the mutants with McDGAT2B expression than in a S. cerevisiae mutant with endogenous DGA1 expression. McDGAT2B prefers saturated fatty acids to monounsaturated fatty acids and has an obvious preference for C18:3 (ω-6) according to the results of substrate preference experiments. Furthermore, only the mRNA expression pattern of McDGAT2B correlated with TAG biosynthesis during a fermentation process. Our experiments strongly indicate that McDGAT2B is crucial for TAG accumulation, suggesting that it may be an essential target for metabolic engineering aimed at increasing lipid content of M. circinelloides.

中文翻译：

油脂性真菌Mucor circinelloides中的二酰基甘油酰基转移酶的鉴定与表征

酰基辅酶A：二酰基甘油酰基转移酶（DGAT）是三酰基甘油（TAG）合成的关键调节剂。产油性真菌Mucor circinelloides具有四个推定的DGAT：McDGAT1A，McDGAT1B，McDGAT2A和McDGAT2B，分别分为DGAT1和DGAT2子家族。为鉴定并特征分析中DGATs M.卷枝，这四个基因在表达酿酒酵母H1246（TAG缺陷型四重突变体），单独地。TAG生物合成仅通过McDGAT2B的表达得以恢复，并且具有McDGAT2B表达的突变体中的TAG含量明显高于酿酒酵母。具有内源性DGA1表达的突变体。根据底物偏爱实验的结果，McDGAT2B比单不饱和脂肪酸更喜欢饱和脂肪酸，并且对C18：3（ω-6）具有明显的偏爱。此外，在发酵过程中，只有McDGAT2B的mRNA表达模式与TAG生物合成相关。我们的实验有力地表明McDGAT2B是TAG积累至关重要，这表明它可能是代谢工程旨在增加脂肪含量的重要指标M.卷枝。

更新日期：2018-01-10

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11