Artificial Cell Fermentation as a Platform for Highly Efficient Cascade Conversion,ACS Synthetic Biology

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Artificial Cell Fermentation as a Platform for Highly Efficient Cascade Conversion
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2017-12-20 00:00:00 , DOI: 10.1021/acssynbio.7b00365
Kei Fujiwara ₁ , Takuma Adachi ₁ , Nobuhide Doi ₁

Affiliation

Because of its high specificity and stereoselectivity, cascade reactions using enzymes have been attracting attention as a platform for chemical synthesis. However, the sensitivity of enzymes outside their optimum conditions and their rapid decrease of activity upon dilution are drawbacks of the system. In this study, we developed a system for cascade enzymatic conversion in bacteria-shaped liposomes formed by hypertonic treatment, and demonstrated that the system can overcome the drawbacks of the enzymatic cascade reactions in bulk. This system produced final products at a level equivalent to the maximum concentration of the bulk system (0.10 M, e.g., 4.6 g/L), and worked even under conditions where enzymes normally lose their function. Under diluted conditions, the conversion rate of the artificial cell system was remarkably higher than that in the bulk system. Our results indicate that artificial cells can behave as a platform to perform fermentative production like microorganisms.

中文翻译：

人工细胞发酵作为高效级联转化的平台

由于其高特异性和立体选择性，使用酶的级联反应作为化学合成的平台已引起关注。然而，酶在其最佳条件之外的敏感性以及它们在稀释时活性的迅速降低是该系统的缺点。在这项研究中，我们开发了一种通过高渗处理形成的细菌形脂质体中级联酶促转化的系统，并证明了该系统可以克服整体上酶促级联反应的弊端。该系统生产的最终产品水平等于散装系统的最大浓度（0.10 M，例如（4.6克/升），甚至在酶通常失去功能的条件下也能正常工作。在稀释条件下，人造细胞系统的转化率明显高于本体系统。我们的结果表明，人造细胞可以像微生物一样充当发酵生产的平台。

更新日期：2017-12-20

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