Buffer-free integrative nanofluidic device for real-time continuous flow bioassays by ion concentration polarization†,Lab on a Chip

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Buffer-free integrative nanofluidic device for real-time continuous flow bioassays by ion concentration polarization†
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-12-20 00:00:00 , DOI: 10.1039/c7lc01066d
Dinh-Tuan Phan _{1,

2,

2,

3,

4} , Lin Jin _{1,

2,

3} , Shofarul Wustoni _{1,

2,

3} , Chia-Hung Chen _{1,

2,

2,

3,

4}

Affiliation

To perform precision medicine in real-time, a sensor capable of continuously monitoring target biomolecules secreted from a patient under dynamic situations is essential. In this study, a novel portable device combining an aptamer probe and a nanofluidic component was developed, enabling the buffer-free continuous monitoring of small molecules in biological fluids. This integration is synergistic: the aptamer sensor is used to bind target biomolecules, triggering a fluorescence signal change, while the nanofluidic component is applied to achieve ion concentration polarization and convert serum into a clean buffer for aptamer signal regeneration. To demonstrate the system's versatility, we measured various adenosine triphosphate concentrations in human serum for hours with high sensitivity and specificity at minute temporal resolution. Our results demonstrate that this integrative device can be applied for the continuous measurement of target biomolecules and online signal regeneration in patient samples without the use of bulky clean buffer solutions for dynamic real-time healthcare.

中文翻译：

通过离子浓度极化进行实时连续流生物测定的无缓冲集成纳米流体装置†

为了实时进行精确医学，必不可少的传感器能够在动态情况下连续监测从患者体内分泌的目标生物分子。在这项研究中，开发了一种新型的便携式设备，该设备将适体探针和纳米流体组件相结合，可以无缓冲地连续监测生物流体中的小分子。这种整合是协同的：适体传感器用于结合目标生物分子，触发荧光信号变化，而纳米流体成分则用于实现离子浓度极化，并将血清转化为干净的缓冲液以进行适体信号再生。为了证明该系统的多功能性，我们以微小的时间分辨率，以高灵敏度和特异性测量了人类血清中各种浓度的三磷酸腺苷浓度达数小时。

更新日期：2017-12-20

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