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Stable star polymer nanolayers and their thermoresponsiveness as a tool for controlled culture and detachment of fibroblast sheets†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-12-19 00:00:00 , DOI: 10.1039/c7tb02748f Barbara Mendrek 1, 2, 3, 4 , Iwona Żymełka-Miara 1, 2, 3, 4 , Łukasz Sieroń 4, 5, 6, 7, 8 , Agnieszka Fus 4, 5, 6, 7, 8 , Katarzyna Balin 4, 9, 10, 11, 12 , Jerzy Kubacki 4, 9, 10, 11, 12 , Mario Smet 13, 14, 15, 16 , Barbara Trzebicka 1, 2, 3, 4 , Aleksander L. Sieroń 4, 5, 6, 7, 8 , Agnieszka Kowalczuk 1, 2, 3, 4
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-12-19 00:00:00 , DOI: 10.1039/c7tb02748f Barbara Mendrek 1, 2, 3, 4 , Iwona Żymełka-Miara 1, 2, 3, 4 , Łukasz Sieroń 4, 5, 6, 7, 8 , Agnieszka Fus 4, 5, 6, 7, 8 , Katarzyna Balin 4, 9, 10, 11, 12 , Jerzy Kubacki 4, 9, 10, 11, 12 , Mario Smet 13, 14, 15, 16 , Barbara Trzebicka 1, 2, 3, 4 , Aleksander L. Sieroń 4, 5, 6, 7, 8 , Agnieszka Kowalczuk 1, 2, 3, 4
Affiliation
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In this study, we describe novel thermoresponsive star copolymer surfaces used for the first time for the culture of fibroblast sheets, followed by their detachment, controlled by a change in temperature. To date, no star polymers, or their layers, have been used for this purpose. A “grafting to” strategy was applied to obtain poly[oligo(ethylene glycol) methacrylate] star layers on functionalized solid supports. Atom transfer radical polymerization of oligo(ethylene glycol) methacrylates and glycidyl methacrylate initiated with modified poly(arylene oxindole) yielded stars with molar masses up to Mn = 380 000 g mol−1. Stars were attached to a glass substrate via the reaction between the functional epoxy groups of the stars with the amine groups of the functionalized substrate. The thickness of the layer was related to the dimensions of isolated stars in solution, which showed that multilayers were obtained. Above the phase transition temperature, polymer nanolayers were hydrophobic, thus enabling the growth of fibroblasts on their surfaces and the formation of a cell sheet. Decreasing the temperature below the phase transition temperature made the star surfaces hydrophilic. This eliminated the affinity of the surface for cells and led to detachment of the intact fibroblast sheet. These observations have shown for the first time that the star polymer architecture favors the detachment of cell sheets as compared to linear polymer analogues grafted onto supports, thus reducing the time of this process. Knowledge of the influence of the polymer topology on layer properties and cell growth and detachment can aid in the development of polymeric materials for tissue culture applications.
中文翻译：
稳定的星形聚合物纳米层及其热响应性，可作为控制培养和分离成纤维细胞片的工具†
在这项研究中，我们描述了新颖的热响应性星形共聚物表面，该表面首次用于培养成纤维细胞片，然后通过温度变化控制其分离。迄今为止，还没有星形聚合物或其层被用于此目的。应用了“接枝到”策略，以在功能化固体支持物上获得聚[低聚（乙二醇）甲基丙烯酸甲酯]星形层。用改性的聚（亚芳基氧吲哚）引发的低聚甲基丙烯酸乙二醇酯和甲基丙烯酸缩水甘油酯的原子转移自由基聚合产生的摩尔质量高达M n = 380 000 g mol -1。恒星通过恒星的官能环氧基与官能化底物的胺基之间的反应。该层的厚度与溶液中孤立星的尺寸有关，这表明获得了多层。在相变温度以上，聚合物纳米层是疏水的，因此能够使成纤维细胞在其表面上生长并形成细胞片。将温度降低至相变温度以下使星形表面具有亲水性。这消除了表面对细胞的亲和力，并导致完整的成纤维细胞片脱离。这些观察结果首次表明，与接枝到支持物上的线性聚合物类似物相比，星形聚合物结构有利于细胞片的分离，从而减少了该过程的时间。
更新日期：2017-12-19
中文翻译：
稳定的星形聚合物纳米层及其热响应性，可作为控制培养和分离成纤维细胞片的工具†
在这项研究中，我们描述了新颖的热响应性星形共聚物表面，该表面首次用于培养成纤维细胞片，然后通过温度变化控制其分离。迄今为止，还没有星形聚合物或其层被用于此目的。应用了“接枝到”策略，以在功能化固体支持物上获得聚[低聚（乙二醇）甲基丙烯酸甲酯]星形层。用改性的聚（亚芳基氧吲哚）引发的低聚甲基丙烯酸乙二醇酯和甲基丙烯酸缩水甘油酯的原子转移自由基聚合产生的摩尔质量高达M n = 380 000 g mol -1。恒星通过恒星的官能环氧基与官能化底物的胺基之间的反应。该层的厚度与溶液中孤立星的尺寸有关，这表明获得了多层。在相变温度以上，聚合物纳米层是疏水的，因此能够使成纤维细胞在其表面上生长并形成细胞片。将温度降低至相变温度以下使星形表面具有亲水性。这消除了表面对细胞的亲和力，并导致完整的成纤维细胞片脱离。这些观察结果首次表明，与接枝到支持物上的线性聚合物类似物相比，星形聚合物结构有利于细胞片的分离，从而减少了该过程的时间。
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