A lysosome targetable versatile fluorescent probe for imaging viscosity and peroxynitrite with different fluorescence signals in living cells†,Journal of Materials Chemistry B

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A lysosome targetable versatile fluorescent probe for imaging viscosity and peroxynitrite with different fluorescence signals in living cells†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-12-19 00:00:00 , DOI: 10.1039/c7tb02615c
Bingpeng Guo _{1,

2,

3,

4,

5} , Jing Jing _{1,

2,

3,

4,

5} , Longxue Nie _{1,

2,

3,

4,

5} , Fangyun Xin _{1,

2,

3,

4,

5} , Congcong Gao _{1,

2,

3,

4,

5} , Wen Yang _{1,

2,

3,

4,

5} , Xiaoling Zhang _{1,

2,

3,

4,

5}

Affiliation

The lysosome, which acts as the cellular recycling centre, is filled with numerous hydrolases that can degrade most cellular macromolecules. The abnormalities of the lysosome are closely associated with diseases, such as Heřmanský-Pudlák syndrome, Griscelli syndrome and Chédiak-Higashi syndrome. Studies have shown that abnormal viscosity and the accumulation of reactive oxygen species (ROS) in the lysosome will disorder the normal function of the lysosome. In this research, a versatile fluorescent probe Lyso-NA has been developed for the multi-channel imaging of lysosomal viscosity and peroxynitrite (ONOO⁻). When excited at 550 nm, the Lyso-NA exhibited about a 50-fold increase in fluorescence at 610 nm and also with the increasing viscosity from 1.0 cP to 1410 cP, and about a 3.5-fold increase in fluorescence at 510 nm (excitation at 440 nm) together with the increasing ONOO⁻. These satisfactory response properties make it possible to use Lyso-NA to monitor changes in both viscosity and ONOO⁻ inside the lysosome. To achieve its practical application, it was further demonstrated that Lyso-NA exhibits low cytotoxicity, and good cell permeability, and could be used to monitor lysosomal viscosity and ONOO⁻ in living cells.

中文翻译：

溶酶体可靶向多功能荧光探针，用于在活细胞中对具有不同荧光信号的粘度和过氧亚硝酸盐进行成像†

溶酶体充当细胞回收中心，充满了许多可降解大多数细胞大分子的水解酶。溶酶体的异常与疾病密切相关，例如Heřmanský-Pudlák综合征，Griscelli综合征和Chédiak-Higashi综合征。研究表明，异常的粘度和溶酶体中活性氧（ROS）的积累会扰乱溶酶体的正常功能。在这项研究中，一个通用的荧光探针溶血NA已被开发用于溶酶体粘度的多通道成像和过氧亚硝酸盐（ONOO ^-）。当在550 nm激发时，Lyso-NA显示约在荧光在610nm处并且还1410厘泊50倍的增加与增加的粘度为1.0厘泊，约在510nm（激发在440nm）一起在荧光的3.5倍的增加与ONOO增加^-。这些令人满意的响应特性使之能够使用溶血NA监测粘度和ONOO变化^-溶酶体内。为了实现其实际应用，它进一步证明溶血NA表现出低细胞毒性，和良好的细胞渗透性，并且可以用于监测溶酶体粘度和ONOO ^-在活细胞。

更新日期：2017-12-19

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