Fish are currently used for the assessment of chemical toxicity. The REACh regulation and the European directive on the protection of animals used for scientific purposes both recommend the use of methods other than animal testing. In view of this, fish cell lines are increasingly used to provide fast and reliable toxic and ecotoxic data on new chemicals. The sensitivity of the Rainbow trout liver cell line RTL-W1 and Japanese medaka embryos cell line OLCAB-e3 were used with different toxicity endpoints, namely cytotoxicity, EROD activity, ROS production and DNA damage for various classes of pollutants displaying different modes of action but also with complex environmental mixtures. Toxicity tests were coupled with chemical analysis to quantify the chemical concentrations in cell cultures. Differences in sensitivity were found between fish cell lines. MTT reduction assay revealed that OLCAB-e3 cells were more sensitive than RTL-W1 cells. On the contrary, RTL-W1 gave higher response levels for the Fpg-modified comet assay and ROS assay. The OLCAB-e3 cell line did not express EROD activity unlike RTL-W1. This study highlights the capacity of the two different fish cell lines to measure the toxicity of individual toxicants but also environmental mixtures. Then, results obtained here illustrate the interest of using different cell lines and toxicity endpoints to assess the toxicity of complex or unknown mixture of chemicals.

目前，鱼被用于评估化学毒性。REACh法规和有关用于科学目的的动物保护的欧洲指令均建议使用动物测试以外的方法。有鉴于此，鱼细胞系越来越多地用于提供有关新化学品的快速可靠的有毒和生态毒性数据。虹鳟鱼肝细胞RTL-W1细胞和日本高胚胎细胞OLCAB-e3细胞具有不同的毒性终点，即对各种表现出不同作用方式的污染物，其细胞毒性，EROD活性，ROS产生和DNA损伤的敏感性不同。以及复杂的环境混合物。毒性测试与化学分析相结合，以量化细胞培养物中的化学浓度。在鱼细胞系之间发现了敏感性差异。MTT还原试验表明，OLCAB-e3细胞比RTL-W1细胞更敏感。相反，对于Fpg修饰的彗星测定法和ROS测定法，RTL-W1给出了更高的响应水平。与RTL-W1不同，OLCAB-e3细胞系不表达EROD活性。这项研究强调了两种不同鱼类细胞系测量单个有毒物质以及环境混合物的毒性的能力。然后，此处获得的结果说明了使用不同的细胞系和毒性终点来评估复杂或未知化学混合物的毒性的兴趣。与RTL-W1不同，OLCAB-e3细胞系不表达EROD活性。这项研究强调了两种不同鱼类细胞系测量单个有毒物质以及环境混合物的毒性的能力。然后，此处获得的结果说明了使用不同的细胞系和毒性终点来评估复杂或未知化学混合物的毒性的兴趣。与RTL-W1不同，OLCAB-e3细胞系不表达EROD活性。这项研究强调了两种不同鱼类细胞系测量单个有毒物质以及环境混合物的毒性的能力。然后，此处获得的结果说明了使用不同的细胞系和毒性终点来评估复杂或未知化学混合物的毒性的兴趣。