Cytochrome P450 (CYP450) enzymes are a family of hemoproteins primarily responsible for detoxification functions. Earthworms have been used as a bioindicator of soil pollution in numerous studies, but no CYP450 gene has so far been cloned. RT-PCR and RACE-PCR were employed to construct and sequence the CYP450 gene DNA from the extracted mRNA in the earthworm Eisenia fetida. The cloned gene (EW1) has an open reading frame of 477 bp. The 3′-terminal region contained both the consensus and the signature sequences characteristic of CYP450. It was closely related to the CYP450 gene from the flatworm genus Opisthorchis felineus with 87% homology. The predicted structure of the putative protein was 97% homologous to human CYP450 family 27. This gene has been deposited in GenBank (accession no. KM881474). Earthworms (E. fetida) were then exposed to 1, 10, 100, and 500 mg kg⁻¹ enrofloxacin in soils to explore the mRNA expression by real time qPCR. The effect of enrofloxacin on mRNA expression levels of EW1 exhibited a marked hormesis pattern across the enrofloxacin dose range tested. This is believed to be the first reported CYP450 gene in earthworms, with reference value for molecular studies on detoxification processes in earthworms.

细胞色素P450（CYP450）酶是主要负责排毒功能的血红蛋白家族。在许多研究中，s已被用作土壤污染的生物指示剂，但迄今为止尚未克隆出CYP450基因。用RT-PCR和RACE-PCR从toEisenia fetida提取的mRNA中构建CYP450基因DNA并进行测序。克隆的基因（EW1）的开放阅读框为477 bp。3'末端区域包含CYP450特征性的共有序列和特征序列。它与扁豆虫猫（Oisthorchis felineus）的CYP450基因密切相关。具有87％的同源性。推定蛋白质的预测结构与人CYP450家族27的同源性为97％。该基因已保存在GenBank中（登录号KM881474）。然后将f（E。fetida）暴露于土壤中的1、10、100和500 mg kg ^-1恩诺沙星中，以通过实时qPCR探索mRNA的表达。在测试的恩诺沙星剂量范围内，恩诺沙星对EW1 mRNA表达水平的影响表现出明显的兴奋作用模式。据信这是earth中第一个被报道的CYP450基因，对molecular的解毒过程的分子研究具有参考价值。