The application of the herbicide chlorimuron-ethyl has a lasting toxic effect on some succession crops. Here, a bacterium capable of utilizing chlorimuron-ethyl as the sole source of nitrogen was isolated from the contaminated soil and was identified as Enterobacter ludwigii sp. CE-1, and its detoxification and degradation of the herbicide were then examined. The biodegradation of chlorimuron-ethyl by the isolate CE-1 was significantly accelerated with increasing concentration (1–10 mg/l) and temperature (20–40 °C). The optimal pH for the degradation of chlorimuron-ethyl by the isolate CE-1 was pH 7.0. A pathway for the biodegradation of chlorimuron-ethyl by the isolate CE-1 was proposed, in which it could be first converted into 2-amino-4-chloro-6-methoxypyrimidine and an intermediate product by the cleavage of the sulfonylurea bridge and then transformed into saccharin via hydrolysis and amidation. The plant height and fresh weight of corn that had been incubated in nutrient solution containing 0.2 mg/l of chlorimuron-ethyl significantly recovered to 83.9% and 83.1% compared with those in the uninoculated control, although the root growth inhibition of chlorimuron-ethyl could not be alleviated after inoculation for 14 d. The results indicate that the isolate CE-1 is a promising bacterial resource for the biodegradation and detoxification of chlorimuron-ethyl.

除草剂氯嘧磺隆对某些演替作物具有持久的毒性作用。在这里，从受污染的土壤中分离出一种能够利用氯嘧磺隆作为唯一氮源的细菌，并将其鉴定为路德维希肠杆菌。sp。然后检查CE-1及其除草剂的解毒和降解。随着浓度（1–10 mg / l）和温度（20–40°C）的升高，CE-1菌株对嘧磺隆的生物降解作用显着加快。分离物CE-1降解氯嘧磺隆的最佳pH为7.0。提出了一种通过CE-1分离菌对氯嘧磺隆进行生物降解的途径，在该途径中，首先可以通过磺酰脲桥的裂解将其转化为2-氨基-4-氯-6-甲氧嘧啶及其中间体。通过水解和酰胺化转化为糖精。与未接种对照相比，在含有0.2 mg / l氯嘧磺隆的营养液中培养的玉米的株高和鲜重显着恢复到83.9％和83.1％，接种14 d后，氯嘧磺隆的根系生长抑制作用没有减轻。结果表明，分离物CE-1是对氯嘧磺隆进行生物降解和解毒的有前途的细菌资源。