Despite intense research on the in vitro characterization of regulatory factors modulating the alternative oxidase (AOX) pathway, the regulation of its activity in vivo is still not fully understood. Advances concerning in vivo regulation of AOX based on the oxygen-isotope fractionation technique are reviewed, and regulatory factors that merit future research are highlighted. In addition, we review and discuss the main biological functions assigned to the plant AOX, and suggest future experiments involving in vivo activity measurements to test different hypothesized physiological roles.

中文翻译：

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一个体内的交替氧化酶途径中的作用（S）的视角

尽管对调节替代氧化酶（AOX）途径的调节因子的体外表征进行了深入研究，但对其在体内活性的调节仍未完全了解。综述了基于氧同位素分馏技术的AOX体内调控研究进展，并提出了值得进一步研究的调控因素。此外，我们审查并讨论了分配给植物AOX的主要生物学功能，并建议了涉及体内活性测量的未来实验，以测试不同的假设生理作用。