Abstract The effects of ultrasound (400 W, for 0, 20, 40, 60, 80, or 100 min) pre-treatment on the physicochemical properties, functionality and structure of laccase-catalyzed α-LA in the presence of ferulic acid (FA), were investigated in this study. Size exclusion chromatography showed that α-LA monomers decreased significantly in the presence of laccase and FA, and its high molecule polymers were formed with increasing ultrasonic time. SDS-PAGE analysis also showed that ultrasound treatment contributed to the formation of oligomers and polymers of α-LA treated with laccase and FA. Surface hydrophobility and gel strength of laccase-catalyzed α-LA-FA conjugates were higher than that of three control systems, and gradually enhanced with the ultrasonic time increased. Moreover, ultrasound-treated α-LA with laccase and FA had greater G′ values than other three control systems according to the rheological measurements. Ultrasound treatment did not significantly changed conformational structure of laccase-catalyzed α-LA-FA conjugates, as demonstrated by CD spectra. Therefore, ultrasound contributed to form greater non-covalent and covalent interactions between α-LA molecules and aggregates, and could remarkably change physicochemical and functional properties of laccase-catalyzed α-LA-FA conjugates.

中文翻译：

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超声对漆酶催化α-乳清蛋白结构和功能特性的影响

摘要 在阿魏酸 (FA) 存在下, 超声 (400 W, 0, 20, 40, 60, 80, 或 100 min) 预处理对漆酶催化的 α-LA 理化性质、功能和结构的影响)，在本研究中进行了调查。尺寸排阻色谱表明，α-LA单体在漆酶和FA存在下显着减少，随着超声时间的增加，其高分子聚合物形成。SDS-PAGE 分析还表明，超声处理有助于形成用漆酶和 FA 处理的 α-LA 的低聚物和聚合物。漆酶催化的α-LA-FA偶联物的表面疏水性和凝胶强度高于三个对照体系，并随着超声时间的增加而逐渐增强。而且，根据流变学测量，用漆酶和 FA 超声处理的 α-LA 具有比其他三个对照系统更大的 G' 值。如 CD 光谱所示，超声处理没有显着改变漆酶催化的 α-LA-FA 偶联物的构象结构。因此，超声有助于在 α-LA 分子和聚集体之间形成更大的非共价和共价相互作用，并且可以显着改变漆酶催化的 α-LA-FA 偶联物的理化和功能特性。