Label-free detection of fibrinogen based on the fibrinogen-enhanced peroxidase activity of a fibrinogen–hemin composite†,Analyst

当前位置： X-MOL 学术 › Analyst › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Label-free detection of fibrinogen based on the fibrinogen-enhanced peroxidase activity of a fibrinogen–hemin composite†
Analyst ( IF 3.6 ) Pub Date : 2017-12-19 00:00:00 , DOI: 10.1039/c7an01661a
Tingting Hou _{1,

2,

3,

4,

5} , Yuanfu Zhang _{1,

2,

3,

4,

5} , Tao Wu _{1,

2,

3,

4,

5} , Meifeng Wang _{1,

2,

3,

4,

5} , Yinghong Zhang _{1,

2,

3,

4,

5} , Rui Li _{1,

2,

3,

4,

5} , Lei Wang _{1,

2,

3,

4,

5} , Qingwang Xue _{1,

2,

3,

4,

5} , Shuhao Wang _{1,

2,

3,

4,

5}

Affiliation

A simple, label-free colorimetric method for the determination of fibrinogen (Fib) in plasma is presented. In this work, it was observed that Fib interacted with hemin to form a hemin–Fib composite. Because Fib prevented hemin from the formation of m-oxo-dimers, the hemin–Fib composite possesses excellent peroxidase-like activity. Importantly, the peroxidase-like activity of Fib–hemin increased with the increase in the Fib. This allows us to utilize the H₂O₂–ABTS colorimetric system for the quantitative analysis of Fib. This optimized method provided a linear determination range of 2.0–100 pM with a correlation of 0.9975. The limit of detection for Fib was experimentally determined to be 0.7 pM based on a signal-to-noise ratio (S/N) of 3. This novel approach provides a rapid, sensitive, cost efficient and robust bioassay for detection of Fib in pathology and clinical applications.

中文翻译：

基于无标记检测纤维蛋白原的纤维蛋白原增强过氧化物酶纤维蛋白原复合氯化血红素的活性†

提出了一种简单，无标记的比色法测定血浆中的纤维蛋白原（Fib）。在这项工作中，观察到Fib与血红素相互作用形成了Hemin-Fib复合物。因为Fib阻止了血红素形成m-氧代二聚体，所以hemin-Fib复合物具有出色的过氧化物酶样活性。重要的是，Fib-hemin的过氧化物酶样活性随Fib的增加而增加。这使我们可以利用H ₂ O ₂–ABTS比色系统，用于对Fib进行定量分析。这种优化的方法提供了2.0-100 pM的线性测定范围，相关系数为0.9975。根据3的信噪比（S / N），实验确定Fib的检出限为0.7 pM。这种新方法为病理学检测Fib提供了一种快速，灵敏，经济高效且可靠的生物测定方法和临床应用。

更新日期：2017-12-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11