We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU⁻¹, which supports a limit of detection of 3.2 ng mL⁻¹ for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.

中文翻译：

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使用嵌入了无标记生物传感器的微嵌入式微流控芯片，从全血中进行实时CRP检测†

我们展示了通过使用由微流控芯片和无标记生物传感器组成的芯片实验室系统，无需样品预处理即可从全血样品中检测C-创意蛋白（CRP）。微流控芯片包括用于过滤血细胞的微柱阵列，并且仅允许血浆流过并到达引导模式共振（GMR）生物传感器以进行实时监控。研发的GMR传感器可实现186 nm RIU ^-1的整体灵敏度，支持3.2 ng mL ^-1的检测限用于在人血清中加标的重组CRP。结果与使用酶联免疫吸附测定获得的结果相当。此外，我们证明了使用微管柱过滤血细胞并通过使用全血和血浆样品使用GMR传感器同时测量CRP浓度的功效。