A pH responsive AIE probe for enzyme assays†,Analyst

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A pH responsive AIE probe for enzyme assays†
Analyst ( IF 3.6 ) Pub Date : 2017-12-19 00:00:00 , DOI: 10.1039/c7an01710c
Leilei Shi _{1,

2,

3,

4,

5} , Yufeng Liu _{1,

2,

3,

4,

5} , Qian Wang _{1,

2,

3,

4,

5} , Tiankuo Wang _{3,

4,

5,

6,

7} , Yubin Ding _{1,

2,

3,

4,

5} , Yi Cao _{3,

4,

5,

6,

7} , Zhe Li _{1,

2,

3,

4,

5} , Hui Wei _{1,

2,

3,

4,

5}

Affiliation

By combining leucine (Leu) and tetraphenylethene (TPE), a pH-sensitive aggregation induced emission (AIE) probe TPE-Leu was developed. The aliphatic amine in TPE-Leu was more easily protonated under acidic conditions, which made TPE-Leu more water soluble. Therefore, the protonated AIE probe showed weak fluorescence under acidic conditions. When the pH was changed to basic conditions, it showed strong fluorescence due to the hydrophobic nature of TPE-Leu. We demonstrated that the probe showed high selectivity toward pH changes with the coexistence of other potential species such as metal ions, redox agents, and biomolecules. In contrast, TPE-NH₂ did not exhibit obvious pH-sensitive properties. Moreover, TPE-Leu was further utilized to develop a sensitive and selective sensing platform for urease and acetylcholinesterase (AChE) detection. The current study not only provides a new strategy for designing pH-sensitive fluorescent probes for bioassays but also broadens the applications of AIE probes.

中文翻译：

用于酶分析的pH响应型AIE探针†

通过将亮氨酸（Leu）和四苯乙烯（TPE）结合，开发了一种pH敏感的聚集诱导发射（AIE）探针TPE-Leu。TPE-Leu中的脂肪胺在酸性条件下更容易质子化，这使TPE-Leu的水溶性更高。因此，质子化的AIE探针在酸性条件下显示弱荧光。当pH值更改为碱性条件时，由于TPE-Leu的疏水性，它显示出强荧光。我们证明了该探针与其他潜在物种（例如金属离子，氧化还原剂和生物分子）共存时，对pH值变化表现出很高的选择性。相比之下，TPE-NH ₂没有表现出明显的pH敏感特性。此外，TPE-Leu进一步用于开发灵敏和选择性的传感平台，用于尿素酶和乙酰胆碱酯酶（AChE）检测。当前的研究不仅为设计用于生物分析的pH敏感的荧光探针提供了新的策略，而且拓宽了AIE探针的应用范围。

更新日期：2017-12-19

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