High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor†,Chemical Science

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High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor†
Chemical Science ( IF 7.6 ) Pub Date : 2017-12-19 00:00:00 , DOI: 10.1039/c7sc05167k
Ngong Kodiah Beyeh _{1,

2} , Isabel Díez ₁ , S Maryamdokht Taimoory ₂ , Daniel Meister ₂ , Andrew I Feig ₃ , John F Trant ₂ , Robin H A Ras _{1,

4} , Kari Rissanen ₅

Affiliation

Pyrophosphate (PPi) is a byproduct of DNA and RNA synthesis, and abnormal levels are indicative of disease. We report the high-affinity binding of PPi in water by N-alkyl ammonium resorcinarene chloride receptors. Experimental analysis using ¹H and ³¹P NMR, isothermal titration calorimetry, mass spectrometry, and UV-vis spectroscopy all support exceptional selectivity of these systems for PPi in water. The measured affinity of K₁ = 1.60 × 10⁷ M⁻¹ for PPi is three orders of magnitude larger than that observed for binding to another phosphate, ATP. This exceptional anion-binding affinity in water is explored through a detailed density functional theory computational study. These systems provide a promising avenue for the development of future innovative medical diagnostic tools.

中文翻译：

通过间苯二酚盐受体对水中的焦磷酸盐进行高亲和力和选择性检测†

焦磷酸盐 (PPi) 是 DNA 和 RNA 合成的副产品，异常水平表明存在疾病。我们报道了N-烷基间苯二酚氯化铵受体对水中 PPi 的高亲和力结合。使用¹ H 和³¹ P NMR、等温滴定量热法、质谱法和紫外-可见光谱进行的实验分析都支持这些系统对水中 PPi 的卓越选择性。测得的 PPi 亲和力K ₁ = 1.60 × 10 ⁷ M ^-1比观察到的与另一种磷酸盐 ATP 结合的亲和力大三个数量级。通过详细的密度泛函理论计算研究探索了水中这种特殊的阴离子结合亲和力。这些系统为未来创新医疗诊断工具的开发提供了一条有前途的途径。

更新日期：2017-12-19

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