“Pyrene-box” cages easily crystallize from aqueous solutions and readily encapsulate compounds of biological interest. These host–guest systems can be obtained under ambient conditions from 1,3,5,8-pyrenetetrasulfonate (PTS), guanidinium derivatives (G⁺) and biogenic guests bearing cationic groups. Out of the many examples of synthetic molecular capsules, Pyrene box cages are completely responding to the requirements of green chemistry: non-toxic, water soluble, cheap, commercially available compounds. The “Pyrene-Box” cages have been used for the in situ encapsulation of unstable or non-crystalline biogenic compounds, allowing the complete molecular structure determination of species that do not crystallize by themselves. The encapsulated guests have a reduced motional degrees of freedom which is obtained via their anchoring to the Pyrene box cages that allows at the same time to reduce a significant amount of disorder. In this highlight we discuss the recent developments of the encapsulation chemistry of the “Pyrene box” and the strategy behind its synthesis, together with further possible developments and the limitations of this biomimetic supramolecular cage system. The “Pyrene Box” shows great potential for future applications, ranging from fundamental studies of structure determination of unstable molecules to drug delivery vehicles.

中文翻译：

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yr盒胶囊，用于不稳定或非晶客体分子的自适应包封和结构确定

“ P盒”笼子很容易从水溶液中结晶出来，并容易地封装具有生物学意义的化合物。这些宿主-客体系统可以在环境条件下从1,3,5,8-re四磺酸盐（PTS），胍衍生物（G ⁺）和带有阳离子基团的生物客体中获得。在许多合成分子胶囊的例子中，P盒笼完全符合绿色化学的要求：无毒，水溶性，便宜，可商购的化合物。“ P盒”笼已用于原位封装不稳定或非结晶的生物化合物，从而可以完全确定自身无法结晶的物种的分子结构。被包囊的客体具有降低的运动自由度，这是通过将其锚定在P盒笼子上而实现的，从而可以同时减少大量的混乱。在这一亮点中，我们讨论了“ P盒”封装化学的最新进展及其合成背后的策略，以及这种仿生超分子笼系统的进一步可能的发展和局限性。“ P盒”显示出巨大的潜在应用前景，从不稳定分子的结构确定的基础研究到药物输送载体。